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Z-WEHD-FMK

Catalog No.
A1924
Caspase 5 inhibitor,potent,cell-permeable and irreversible
Grouped product items
SizePriceStock Qty
1mg
$77.00
In stock
5mg
$231.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Z-WEHD-FMK (CAS 210345-00-9) is a peptide-based inhibitor primarily targeting inflammatory caspases, including caspase-1, -4, and -5. It irreversibly blocks caspase-mediated proteolytic cleavage, interfering with cellular signaling pathways involved in inflammation and apoptosis. Studies demonstrate that Z-WEHD-FMK treatment prevents Chlamydia-induced fragmentation of the Golgi apparatus by inhibiting golgin-84 cleavage, thereby decreasing bacterial proliferation and altering lipid trafficking to pathogen-containing inclusions. This inhibitor is frequently utilized in cell biology and infectious disease research to investigate caspase-related cellular processes and microbial pathogenesis mechanisms.

Product Citation

Chemical Properties

StorageStore at -20°C
M.Wt763.77
Cas No.210345-00-9
FormulaC37H42FN7O10
SynonymsZ-Trp-Glu(OMe)-His-Asp(OMe)-FMK
Solubilityinsoluble in H2O; ≥26.32 mg/mL in EtOH with ultrasonic; ≥46.33 mg/mL in DMSO
Chemical Namemethyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-4-[[(2S)-3-(1H-indol-3-yl)-2-(phenylmethoxycarbonylamino)propanoyl]amino]-5-oxopentanoate
SDFDownload SDF
Canonical SMILESCOC(=O)CCC(C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC2=CNC3=CC=CC=C32)NC(=O)OCC4=CC=CC=C4
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

Chlamydia trachomatis (C. trachomatis)-infected HeLa cells

Reaction Conditions

80 μM Z-WEHD-FMK for 9 h incubation

Applications

Z-WEHD-FMK (80 μM; 9 h) elicited a near-complete blockage of C. trachomatis-induced cleavage of golgin-84 and increased GM130 expression in cells. Z-WEHD-FMK treatment resulted in a 2-log reduction in numbers of infectious bacteria, which could be reversed by knocking down golgin-84. Thus, Z-WEHD-FMK could effectively prevent Golgi fragmentation, and was used to explore the role of fragmentation of the Golgi compartment in Chlamydia reproduction.

Note

The technical data provided above is for reference only.

References:

1. Heuer D, Rejman Lipinski A, Machuy N, et al. Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction. Nature, 2009, 457(7230): 731-735.

Biological Activity

A synthetic peptide that irreversibly inhibits caspase-5 and related caspase activity.
Targets caspase-5          
IC50            

Quality Control

Chemical structure

Z-WEHD-FMK

Related Biological Data

Z-WEHD-FMK