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Z-WEHD-FMK
Caspase 5 inhibitor,potent,cell-permeable and irreversible

Catalog No.A1924
Size Price Stock
1mg
$55.00
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5mg
$150.00
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10mg
$250.00
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25mg
$400.00
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Tel: +1-832-696-8203

Email: [email protected]

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Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Yang D, Zheng X, et al. "Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish." Nat Commun. 2018 Aug 3;9(1):3052. PMID:30076291

Quality Control

Quality Control & MSDS

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Chemical structure

Z-WEHD-FMK

Related Biological Data

Z-WEHD-FMK

Biological Activity

A synthetic peptide that irreversibly inhibits caspase-5 and related caspase activity.
Targets caspase-5          
IC50            

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Chemical Properties

Cas No. 210345-00-9 SDF Download SDF
Synonyms Z-Trp-Glu(OMe)-His-Asp(OMe)-FMK
Chemical Name methyl (4S)-5-[[(2S)-1-[[(3S)-5-fluoro-1-methoxy-1,4-dioxopentan-3-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-4-[[(2S)-3-(1H-indol-3-yl)-2-(phenylmethoxycarbonylamino)propanoyl]amino]-5-oxopentanoate
Canonical SMILES COC(=O)CCC(C(=O)NC(CC1=CN=CN1)C(=O)NC(CC(=O)OC)C(=O)CF)NC(=O)C(CC2=CNC3=CC=CC=C32)NC(=O)OCC4=CC=CC=C4
Formula C37H42FN7O10 M.Wt 763.77
Solubility N/A Storage Store at -20°C
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

Treatment of infected cells with pan-caspase inhibitor IV and Z-WEHD-FMK, an inhibitor of inflammatory caspases, elicited a near-complete blockage of C. trachomatis-induced cleavage of golgin-84. Golgin-84 cleavage was blocked via treatment of infected cells with Z-WEHD-FMK, resulting in a lack of Golgi fragmentation and a 2-log reduction in numbers of infectious bacteria.

We treated infected cells with Z-WEHD-FMK, effectively preventing Golgi fragmentation, or with DMSO as a control and then labelled cells with fluorescent ceramide. Confocal images revealed that ceramide was rapidly incorporated into the inclusion membrane within DMSO-treated cells and accumulated inside the inclusion in bacterial membranes. In contrast, Z-WEHD-FMK-treated cells were only slightly fluorescent as the majority of lipid accumulated in a Golgi-like structure outside the inclusion1.

General caspase inhibitor (Z-Asp-CHz-DCB) and capase-5 inhibitor (Z-WEHD-FMK) could not induce rRNA fragmentation treated with ECyd. Caspase-5 (ICErei Ill/TY), member of ICE protease, activated pathway may be concerned with ECyd induced rRNA fragmentation2.

References:
1. D. Heuer, A.R. Lipinski et al. Chlamydia causes fragmentation of the Golgi compartment to ensure reproduction. NATURE, 457, 2009
2. Kamada, S., Funahashi, Y. and Tsujimoto, Y.(1997) Cell Death Diff. 4, 473-478.