Protease Inhibitor Cocktail (EDTA-Free, 200X in DMSO)

Protease inhibitor cocktails are commonly used in protein extraction to prevent protein breakdown by natural cellular proteases. These enzymes (including cysteine, serine, acid proteases, and aminopeptidases) are activated when cells or tissues are disrupted and can damage target proteins. This ready-to-use 200X DMSO solution uses a mix of inhibitors (AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A) to block such proteases, preserving proteins’ natural structure and properties. Being EDTA-free, it works well for downstream steps sensitive to divalent cations, like phosphorylation analysis or enzyme assays.

When extracting secreted proteins from culture medium in vitro, protein production ceases and degradation increases. Cell crude extracts contain many endogenous enzymes, such as phosphatases and proteases, capable of degrading proteins in the extracts. The best way to improve the yield of intact proteins is to add inhibitors targeting these known enzymes.

The Protease Inhibitor Cocktail is used in tissue culture media to enhance protein stability. It functions by inhibiting proteases responsible for the degradation of both phosphorylated and non-phosphorylated protein substrates. This cocktail is provided as a supplement for tissue culture media to prevent degradation of secreted proteins.

This Protease Inhibitor Cocktail has been optimized and tested specifically for tissue culture media. This protease inhibitor cocktail contains individual components, including Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, aspartic and aminopeptidases. This protease inhibitor cocktail is provided in DMSO as a ready-to-use solution.