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Protease Inhibitor Cocktail (EDTA-Free, 200X in DMSO)

Catalog No.
K1008
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A ready-to-use protease inhibitor cocktail concentrate solution (200X, in DMSO, EDTA-free), equivalent to Sigma P1860, used in tissue culture media to inhibit degradation of secreted proteins.
Grouped product items
SizePriceStock Qty
1ml
$50.00
In stock
20x1ml
$400.00
In stock
50x1ml
$900.00
In stock
100x1ml
$1,350.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

When extracting secreted proteins from culture medium in vitro, protein production ceases and degradation increases. Cell crude extracts contain many endogenous enzymes, such as phosphatases and proteases, capable of degrading proteins in the extracts. The best way to improve the yield of intact proteins is to add inhibitors targeting these known enzymes.

The Protease Inhibitor Cocktail is used in tissue culture media to enhance protein stability. It functions by inhibiting proteases responsible for the degradation of both phosphorylated and non-phosphorylated protein substrates. This cocktail is provided as a supplement for tissue culture media to prevent degradation of secreted proteins.

This Protease Inhibitor Cocktail has been optimized and tested specifically for tissue culture media. This protease inhibitor cocktail contains individual components, including Aprotinin, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, aspartic and aminopeptidases. This protease inhibitor cocktail is provided in DMSO as a ready-to-use solution.

Product Citation

Quality Control

Quality Control & DataSheet

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Protocol

It is suggested to dilute the cocktail appropriately for specific cell lines. The test should begin with at least 200 fold dilution because concentrations of DMSO greater than 0.5% may be deleterious for cells. Since various cell lines will differ in their sensitivity, further dilutions may be needed for this cocktail. This cocktail remains effective for up to 48 hours in the medium. After 48 hours, the medium should be replaced with fresh medium containing the cocktail.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Catalog No. Product Name Summary Targets CAS Number Smiles
A2574 Aprotinin Inhibitor of bovine pancreatic trypsin Proteases|Serine Protease 9087-70-1 CC(O)=O.CC.CC.CCccC.[R].[P].[2H].[L].[E].[P].[P].[Y].[3H].[G].[KH].[R].[R].[Y].F.[Y].[*].[L].F.[V].[Y].[G].[G].[R].[KH].[R].N.N.F.[KH].S.[*].[2H].[R].[G].[G].[*].[KH].[*].[3H].C.[*].F.[P].[*].I.I.N.[Q].[3H].[M]
A2575 Bestatin Aminopeptidase inhibitor Proteases|Aminopeptidase 58970-76-6 CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576 E-64 Cysteine protease inhibitor,irriversible Proteases|Cathepsin 66701-25-5 CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2570 Leupeptin, Microbial Inhibitor of serine and cysteine proteases Proteases|Serine Protease 103476-89-7 O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H]
A2571 Pepstatin A Aspartic proteinases inhibitor Proteases|Other Proteases 26305-03-3 3-hydroxy-4-[2-[[3-hydroxy-6-methyl-4-[[3-methyl-2-[[3-methyl-2-(3-methylbutanoylamino)butanoyl]amino]butanoyl]amino]heptanoyl]amino]propanoylamino]-6-methylheptanoic acid
Stored at -20°C, and stable for at least 12 months.

Storage

Stored at -20°C, and stable for at least 12 months.