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Protease Inhibitor Cocktail (EDTA-Free, 200X in DMSO)

Catalog No.
K1008
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Protease Inhibitor Cocktail
Grouped product items
SizePriceStock Qty
1ml
$50.00
In stock
20x1ml
$400.00
In stock
50x1ml
$900.00
In stock
100x1ml
$1,350.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Protease inhibitor cocktails are commonly used in protein extraction to prevent protein breakdown by natural cellular proteases. These enzymes (including cysteine, serine, acid proteases, and aminopeptidases) are activated when cells or tissues are disrupted and can damage target proteins. This ready-to-use 200X DMSO solution uses a mix of inhibitors (AEBSF, Aprotinin, Bestatin, E-64, Leupeptin, Pepstatin A) to block such proteases, preserving proteins’ natural structure and properties. Being EDTA-free, it works well for downstream steps sensitive to divalent cations, like phosphorylation analysis or enzyme assays.

Product Citation

Quality Control

Quality Control & DataSheet

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Protocol

It is suggested to dilute the cocktail appropriately for specific cell lines. The test should begin with at least 200 fold dilution because concentrations of DMSO greater than 0.5% may be deleterious for cells. Since various cell lines will differ in their sensitivity, further dilutions may be needed for this cocktail. This cocktail remains effective for up to 48 hours in the medium. After 48 hours, the medium should be replaced with fresh medium containing the cocktail.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Catalog No. Product Name Summary Targets CAS Number Smiles
A2574 Aprotinin Inhibitor of bovine pancreatic trypsin Proteases|Serine Protease 9087-70-1 CC(O)=O.CC.CC.CCccC.[R].[P].[2H].[L].[E].[P].[P].[Y].[3H].[G].[KH].[R].[R].[Y].F.[Y].[*].[L].F.[V].[Y].[G].[G].[R].[KH].[R].N.N.F.[KH].S.[*].[2H].[R].[G].[G].[*].[KH].[*].[3H].C.[*].F.[P].[*].I.I.N.[Q].[3H].[M]
A2575 Bestatin Aminopeptidase inhibitor Proteases|Aminopeptidase 58970-76-6 CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576 E-64 Cysteine protease inhibitor,irriversible Proteases|Cathepsin 66701-25-5 CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2570 Leupeptin, Microbial Inhibitor of serine and cysteine proteases Proteases|Serine Protease 103476-89-7 O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H]
A2571 Pepstatin A Aspartic proteinases inhibitor Proteases|Other Proteases 26305-03-3 3-hydroxy-4-[2-[[3-hydroxy-6-methyl-4-[[3-methyl-2-[[3-methyl-2-(3-methylbutanoylamino)butanoyl]amino]butanoyl]amino]heptanoyl]amino]propanoylamino]-6-methylheptanoic acid
Stored at -20°C, and stable for at least 12 months.

Storage

Stored at -20°C, and stable for at least 12 months.