PreScission Protease (PSP)
PreScission Protease (PSP) is a recombinant fusion enzyme consisting of human rhinovirus type 14 (HRV14) 3C protease fused to glutathione S-transferase (GST), expressed using an E. coli expression system. This enzyme selectively recognizes an octapeptide sequence (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro) and catalyzes peptide bond cleavage specifically between the glutamine (Gln) and glycine (Gly) residues. Reaction conditions involve incubation at 4°C in buffer systems optimized for protease activity. PreScission Protease (PSP) is a valuable tool in the field of protein purification, used to cleave recombinant proteins expressed as fusion proteins with this sequence between the vector domain and the target protein.
- 1. Chunyan Feng, Runze Li, et al. "Development of Blocking ELISA Kit Based on Recombinant Monoclonal Antibody for the Serological Diagnosis of Peste Des Petits Ruminants Virus." Journal of AOAC INTERNATIONAL, qsaf077 28 August 2025 PMID: 40875541
- 2. Amanda L. Waterbury, Jonatan Caroli, et al. "Covalent adduct Grob fragmentation underlies LSD1 demethylase-specific inhibitor mechanism of action and resistance." Nat Commun. 2025 Apr 2;16(1):3156. PMID: 40175327
- 3. Megan J R Yeo, Olivia Zhang, et al. "UM171 glues asymmetric CRL3–HDAC1/2 assembly to degrade CoREST corepressors." Nature. 2025 Mar;639(8053):232-240. PMID: 39939761
- 4. Rachana Tomar, Pratibha P Ghodke, et al. "DNA Replication across α-l-(3′-2′)-Threofuranosyl Nucleotides Mediated by Human DNA Polymerase η." Biochemistry. 2024 Oct 1;63(19):2425-2439. PMID: 39259676
- 5. Megan Jr Yeo, Olivia Zhang, et al. "Asymmetric Engagement of Dimeric CRL3KBTBD4 by the Molecular Glue UM171 Licenses Degradation of HDAC1/2 Complexes." bioRxiv. 2024 May 14:2024.05.14.593897. PMID: 38798619
- 6. Michael P. Stone, et al. "Replication Bypass of the N-(2-Deoxy-d-erythro-pentofuranosyl)-urea DNA Lesion by Human DNA Polymerase η." Biochemistry. 2024 Mar 19;63(6):754-766. PMID: 38413007
- 7. Robyn Richie-Jannetta, Pradeep Pallan, et al. "The peroxidation-derived DNA adduct, 6-oxo-M1dG, is a strong block to replication by human DNA polymerase η." J Biol Chem. 2023 Jul 17;105067. PMID: 37468099
- 8. Xiaoying Wei, Zhishuo Wang, et al. "Human TDP1, APE1 and TREX1 repair 3′-DNA–peptide/protein cross-links arising from abasic sites in vitro." Nucleic Acids Res. 2022 Apr 22;50(7):3638-3657. PMID: 35349719
- 9. Jennifer Carlson. "Regulation of epigenetics and development by the xenobiotic/oxidative stress response factor Keap1 in Drosophila." University of Minnesota. July 2022.
Related Biological Data
|
Components |
100 U |
200 U |
500 U |
Storage |
|
PreScission Protease (1 U/μL) |
100 μL |
200 μL |
500 μL |
-80°C |
|
10×Cleavage Buffer |
1 mL |
2x1 mL |
3x1 mL |
-80°C |
|
Shipping: Dry Ice Shelf life: 2 years |
||||
Note: It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles: Store aliquots at -20°C for a validity period of 6 months.
Sterile colorless liquid
Used at low temperatures (4°C)
Specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between gln and gly amino acid residues
With dual tags (N-His & N-GST), easily removable from the sample after cleavage.
