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PreScission Protease (PSP)

Catalog No.
K1101
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HRV 3C protease
Grouped product items
SizePriceStock Qty
100U (1U/uL)
$100.00
In stock
200U (1U/uL)
$140.00
In stock
500U (1U/uL)
$270.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

PreScission Protease (PSP) is a recombinant fusion enzyme consisting of human rhinovirus type 14 (HRV14) 3C protease fused to glutathione S-transferase (GST), expressed using an E. coli expression system. This enzyme selectively recognizes an octapeptide sequence (Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro) and catalyzes peptide bond cleavage specifically between the glutamine (Gln) and glycine (Gly) residues. Reaction conditions involve incubation at 4°C in buffer systems optimized for protease activity. PreScission Protease (PSP) is a valuable tool in the field of protein purification, used to cleave recombinant proteins expressed as fusion proteins with this sequence between the vector domain and the target protein.

Description

PreScission Protease is a recombinant protease consisting of human rhinovirus (HRV) type 14 3C protease fused with GST. This protease specifically recognizes the short peptide sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperatures (4°C) and cleaves between the amino acid residues Gln and Gly. The recognition and cleavage of its substrates depend not only on the primary structure of this recombinant protease, but also on its secondary and tertiary structures. It is specifically used to remove GST tags from exogenous fusion proteins expressed by vectors such as the pGEX-6P series, thereby obtaining target proteins of higher purity.

Product Citation

Quality Control

Quality Control & MSDS

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Components and Storage

Components

K1101-100U

K1101-200U

K1101-500U

Storage

PreScission Protease

100 U

200 U

500 U

-80°C

10X Cleavage Buffer

1 mL

2 x 1 mL

3 x 1 mL

-80°C

Shipping: dry ice                                   Shelf life: 2 years

Note: It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles: store aliquots at -20°C for a validity period of 6 months.

Features

Sterile colorless liquid

Used at low temperatures (4°C)

Specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between gln and gly amino acid residues