PreScission Protease (PSP)

PreScission Protease is a recombinant protease consisting of human rhinovirus (HRV) type 14 3C protease fused with GST. This protease specifically recognizes the short peptide sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperatures (4°C) and cleaves between the amino acid residues Gln and Gly. The recognition and cleavage of its substrates depend not only on the primary structure of this recombinant protease, but also on its secondary and tertiary structures. It is specifically used to remove GST tags from exogenous fusion proteins expressed by vectors such as the pGEX-6P series, thereby obtaining target proteins of higher purity.

Components	K1101-100U	K1101-200U	K1101-500U	Storage
PreScission Protease	100 U	200 U	500 U	-80°C
10X Cleavage Buffer	1 mL	2 x 1 mL	3 x 1 mL	-80°C
Shipping: dry ice                                   Shelf life: 2 years

Note: It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles: store aliquots at -20°C for a validity period of 6 months.

Sterile colorless liquid

Used at low temperatures (4°C)

Specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between gln and gly amino acid residues