PreScission Protease (PSP)
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
PreScission Protease is a recombinant protease that combines human rhinovirus type 14 3C protease and GST. This protease can specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between Gln and Gly amino acid residues. Its substrate recognition and cleavage depend not only on the primary structure of the recombinant protease but also on its secondary structure and tertiary structure. It specifically isolates the heterologous proteins expressed by the vectors such as pGEX-6p series from its fused GST label to obtain heterologous target proteins with higher purity.
Components and Storage
|PreScission Protease||100 U||200 U||500 U|
|10X Cleavage Buffer||1 mL||1 mL x 2||1 mL x 3|
Dry ice transport. -80°C for long-term storage, valid for 2 years; small sub-pack -20°C for 6 months. 10X Cleavage Buffer was stored at -20°C. Avoid repeated freeze-thaw!
Sterile colorless liquid
used at low temperatures (4°C)
specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between gln and gly amino acid residues.