PreScission Protease (PSP)
Catalog No.
K1101
Specifically recognizes and cleaves the short peptide sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro in fusion proteins.
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PreScission Protease is a recombinant protease consisting of human rhinovirus (HRV) type 14 3C protease fused with GST. This protease specifically recognizes the short peptide sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperatures (4°C) and cleaves between the amino acid residues Gln and Gly. The recognition and cleavage of its substrates depend not only on the primary structure of this recombinant protease, but also on its secondary and tertiary structures. It is specifically used to remove GST tags from exogenous fusion proteins expressed by vectors such as the pGEX-6P series, thereby obtaining target proteins of higher purity.
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Components |
K1101-100U |
K1101-200U |
K1101-500U |
Storage |
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PreScission Protease |
100 U |
200 U |
500 U |
-80°C |
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10X Cleavage Buffer |
1 mL |
2 x 1 mL |
3 x 1 mL |
-80°C |
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Shipping: dry ice Shelf life: 2 years |
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Note: It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles: store aliquots at -20°C for a validity period of 6 months.
Sterile colorless liquid
Used at low temperatures (4°C)
Specifically recognize the short peptide Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro at low temperature (4°C) and digest between gln and gly amino acid residues