TEV Protease
TEV protease is a highly specific cysteine protease with exceptional catalytic efficiency. It recognizes the sequence ENLYFQ S, but the amino acid at the P1 position can also be G, A, M, C, or H. TEV protease is commonly used to remove affinity purification tags from fusion proteins, such as Glutathione S-transferase (GST), His, or other tags. As TEV protease itself carries a His-tag, it can easily be removed by nickel affinity resin after the proteolysis reaction.It is suitable for cleavage of His-tagged fusion proteins, since incompletely cleaved His-tagged recombinant protein and the removed His-tagged portion, along with His-tagged TEV protease itself, can all be bound and removed through nickel column chromatography. The desired target protein is obtained in the column flow-through in the meanwhile.
Overnight digestion at 4℃ with TEV protease is recommended. TEV protease retains activity within pH 6.0-9.0, and is significantly reduced or even lost at pH 5.0 or below.
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Components |
1000 U |
5000 U |
10000 U |
Storage |
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TEV Protease |
0.1 mL |
0.5 mL |
1 mL |
-80oC |
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10X TEV Protease Reaction Buffer |
1 mL |
5X 1 mL |
10X 1 mL |
-80 oC |
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0.1 M DTT |
0.5 mL |
2.5 mL |
5 mL |
-80 oC |
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Shipping: Dry Ice Shelf life: 2 years |
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Note: It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles: Store aliquots at -20°C for a validity period of 6 months.