TEV Protease
TEV protease is a cysteine protease used to cleave fusion tags from recombinant proteins.It is expressed using an E. coli expression system. It specifically recognizes the amino acid sequence ENLYFQ▼S, but tolerates amino acids G, A, M, C, or H at the P1' position. TEV protease maintains activity in the pH range of 6.0–9.0, with reduced or absent enzyme activity at pH ≤ 5.0, and is often used in affinity purification steps to remove His, GST, or other fusion tags. To maximize protein activity and structure, cleavage reactions are typically performed overnight at 4°C.
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Components |
1000 U |
5000 U |
10000 U |
Storage |
|
TEV Protease |
0.1 mL |
0.5 mL |
1 mL |
-80°C |
|
10X TEV Protease Reaction Buffer |
1 mL |
5 x 1 mL |
10 x 1 mL |
-80°C |
|
0.1 M DTT |
0.5 mL |
2.5 mL |
5 mL |
-80°C |
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Shipping: dry ice Shelf life: 2 years |
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Note: It is recommended to aliquot upon first use to avoid repeated freeze-thaw cycles: store aliquots at -20°C for a validity period of 6 months.
