GST-tag Protein Purification Kit

Glutathione S-transferase (GST), a key enzyme in glutathione binding reactions, is used in genetic engineering to create GST-tagged fusion proteins. GST-tagged fusion proteins can be efficiently expressed, and easily purified via GST-tag Protein Purification Kit, with elution by excess reduced glutathione (GSH) under mild, non-denaturing conditions, preserving protein activity for structural/functional studies, antibody production, and interaction analyses. GST-tag Protein Purification Kit, offering necessary reagents and empty affinity columns, is simple, rapid, efficient, and highly specific—with lower non-specific binding, stronger pressure tolerance, and more stable GSH conjugation than similar products—capable of purifying up to 10 GST-tagged proteins (max ~12 mg per protein; 4-6 mg for 50 kD proteins), or 500 small purifications without columns.