Active Protein Extraction Reagent (Bacteria)

Active Protein Extraction Reagent (Bacteria) is a gentle, easy-to-use cell lysis reagent consisting of a native detergent containing phosphate buffer, pH 8.0, which effectively destroys cells and dissolves native or recombinant proteins without denaturation. This product is mainly used for direct lysis and extraction of soluble bacterial active proteins expressed by E. coli itself and the expressed recombinant proteins; At the same time, although it cannot be used to solubilize inclusion bodies, it can be used to wash cell debris that removes adhesion from the surface of inclusion bodies to obtain high-purity inclusion body proteins. This product is optimized for use with BL21, DH5ɑ, JM109 and other similar bacteria.
The proteins extracted from this product generally maintain the original structure and biological activity of the protein, and can be used for a variety of biochemical and molecular biology purposes, while being compatible with downstream applications, including affinity chromatography (e.g., fixed metal affinity chromatography, glutathione chromatography), SDS-PAGE and protein quantification (e.g., BCA protein assay), IP, enzyme activity assays, fluorescent protein assays, and more. Depending on the application, protease inhibitors, salts, reducing agents, denaturants, and chelating agents can be added to the reagent.
This product is a special lysis reagent for one-step rapid (15 min) lysis and extraction of bacterial cytoplasmic proteins and intranuclear proteins of nucleated cells. At the same time, compared with common cell disruption methods, such as ultrasonic disruption, high-pressure sterilization, and lysozyme treatment, this product can usually obtain higher yields and higher activity of soluble protein of interest. Products are compatible with Sigma's CelLytic™ series as well as Thermo's B-PERThe TM series is very similar, and the use effect and use are very similar, and in many cases, they can be considered to replace each other.