HyperChrom Glutathione 4FF Agarose
HyperChrom Glutathione 4FF Agarose is an affinity chromatography medium primarily used to purify glutathione S-transferase (GST)-tagged proteins, but also for the purification of other S-transferases or glutathione-dependent proteins. HyperChrom Glutathione 4FF Agarose is the first choice for purifying GST-tagged proteins, with the advantages of high loading, easy regeneration, and easy amplification.
HyperChrom Glutathione 4FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Affinity chromatography media |
|
Ligation |
Glutathione and 10 carbon atoms connect arms |
|
Scaffolding |
4% highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ligand density |
120~320 μmol glutathione/mL chromatography medium |
|
Dynamic load |
≥ 10 mg GST-tagged protein/mL chromatography medium |
|
Flow rates are recommended |
90-300 cm/h |
|
Maximum flow rate |
400 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4 - 30℃ |
|
pH stability * |
3 - 12 |
|
Chemical resistance |
Common aqueous solution, 0.01 M HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol ** |
|
* After the chromatography medium was placed at 40 °C and pH 3-12 for 7 days, its physicochemical properties and functions did not change significantly. ** 30% is v/v, volume ratio. |
|
| Components | PC2003-25 mL | PC2003-100 mL |
| HyperChrom Glutathione 4FF Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||