HyperChrom Ni-NTA FF Agarose
HyperChrom Ni-NTA FF Agarose is an affinity chromatography medium made by chemically coupling the transition metal ion Ni2+ with tetracoordination to a ligand of Nitrilotriacetic acid (NTA) using a highly crosslinked agarose gel as a matrix. It has a very stable octahedral structure, nickel ions are at the center of the octahedron, which protects nickel ions from attack by small molecules, is more stable, and can withstand harsh conditions such as reducing agents, denaturants or couplants in a certain concentration. In addition, due to the pressure resistance of the matrix, which can withstand pressures up to 0.3 MPa, the product can be used for industrial large-scale protein purification, enabling purification of the protein of interest at relatively high flow rates. This chromatography medium uses the interaction between Ni2+ and the side chains of certain amino acids (mainly histidine) on the protein to achieve the separation and purification of proteins containing and not containing these amino acids and containing a large and low number of these amino acids.
HyperChrom Ni-NTA FF Agarose is the first choice for purifying histidine-tagged proteins, with the advantages of high load, easy regeneration, and low cost.
HyperChrom Ni-NTA FF Agarose chromatography media parameters
|
Name |
Description |
|
Chromatography media type |
Affinity chromatography media |
|
Ligation |
NTA-Ni2+ |
|
Scaffolding |
Highly cross-linked agarose |
|
Average particle size |
90 μm |
|
Ligand density |
~15 μmol Ni2+/mL chromatography medium |
|
Dynamic load |
≥40 mg histidine-tagged protein/mL chromatography medium* |
|
Flow rates are recommended |
90-300 cm/h |
|
Maximum flow rate |
400 cm/h |
|
Withstand pressure |
0.3 MPa |
|
Use temperature |
4 - 30℃ |
|
pH stability ** |
2-14 |
|
Chemical resistance |
Common aqueous solution, 0.01 M HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol*** |
|
* Dynamic load measurement conditions: column loading height: 10 cm, test flow rate 150 cm/h; Test buffer: 0.02 M PB, 0.5 M NaCl, 5 mM imidazole, pH 7.4; test sample: tagged protein (1 mg/ml, 6*His); Index: When the protein penetration reaches 10%, the amount of protein loaded per unit volume of media (mL) (mg). **After 7 days of storage of chromatography medium at 40 °C and pH2-14 environment, its physicochemical properties and functions did not change significantly. *** 30% is v/v, volume ratio. |
|
| Components | PC2001-25 mL | PC2001-100 mL |
| HyperChrom Ni-NTA FF Agarose | 25 mL | 100 mL |
Store the components at 4℃ for 5 years. | ||