HyperTrap Glutathione 4FF Column
HyperChrom Glutathione 4FF Agarose is an affinity chromatography medium primarily used to purify glutathione S-transferase (GST)-tagged proteins, but also for the purification of other S-transferases or glutathione-dependent proteins. HyperTrap Glutathione 4FF Column is a ready-to-use chromatography column with HyperChrom Glutathione 4FF Agarose as the filler, which can be used to purify histidine-tagged proteins, with high flow rate, high load, easy regeneration, low cost, easy amplification and so on.
The main body and inner plug of the pre-installed column tube are made of PP, and the inside and outside are polished, and the sieve plate is made of HDPE material, which has excellent physical properties and chemical resistance, corrosion resistance, anti-aging, long life and good safety. 1) Ready-to-use, convenient and fast. 2) Can be used with syringes, peristaltic pumps or chromatography systems. 3) It can be used in series to increase the sample processing capacity.
1. HyperTrap Glutathione 4FF Column preloaded column parameters
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Pre-assembled column specification/name |
1 mL |
5 mL |
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Dimensions (IDxH) |
7.7 x 25 mm |
16 x 25 mm |
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Withstand pressure |
0.3 MPa |
0.3 MPa |
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Workflow Speed* |
1 mL/min |
1-3 mL/min |
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Operating temperature |
4 - 30°C |
4 - 30°C |
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Storage condition |
Store the components at 4℃ for 5 years. |
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*This flow rate is the recommended common flow rate, the pressure resistance, and the use of different types of chromatography packing in the pre-assembled column will be different, please refer to the product manual of the corresponding chromatographic filler. |
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2. HyperTrap Glutathione 4FF Column chromatography media parameters
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Name |
Description |
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Chromatography media type |
Affinity chromatography media |
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Ligation |
Glutathione and 10 carbon atoms connect arms |
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Scaffolding |
4% highly cross-linked agarose |
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Average particle size |
90 μm |
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Ligand density |
120~320 μmol glutathione/mL chromatography medium |
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Dynamic load |
≥ 10 mg GST-tagged protein/mL chromatography medium |
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Flow rates are recommended |
90-300 cm/h |
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Maximum flow rate |
400 cm/h |
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Withstand pressure |
0.3 MPa |
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Use temperature |
4 - 30℃ |
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pH stability * |
3 - 12 |
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Chemical resistance |
Common aqueous solution, 0.01 M HCl, 0.1 M NaOH, 8 M urea, 6 M guanidine hydrochloride, 30% isopropanol ** |
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* After the chromatography medium was placed at 40 °C and pH 3-12 for 7 days, its physicochemical properties and functions did not change significantly. ** 30% is v/v, volume ratio. |
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