Toggle Nav
Close
  • Menu
  • Setting

GST Colorimetric Activity Assay Kit

Catalog No.
K2108
 
Grouped product items
SizePriceStock Qty
100 assays
$1,407.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Glutathione S-transferase (GST) is a group of enzymes that involved in the detoxification of xenobiotics. GST catalyzes the formation of the thiol group of glutathione to electrophilic xenobitocs. It utilizes glutathione to scavenge toxic substances that produced by oxidative stress. The GST Colorimetric Activity Assay Kit is rely on the GST-catalyzed reaction of GSH and the GST substrate-CDNB (1-chloro-2,4-dinitrobenzene). A dinitrophenyl thioether is produced by GST-catalyzed formation of GS-DNB. It can be detected by spectrophotometer at 340 nm. The kit can detect GST activity in crude cell lysate, purified protein fraction and quantitate GST-tagged fusion protein.

Figure 1: Typical GST kinetic assay curve for sample (left) and background (right)
GST activity = (0.1093-0.0067)*0.036*100/0.002 =185 mU/mL

Quality Control

Quality Control & DataSheet

View current batch:
 

Components and Storage

Components

100 Assays

Storage

GST Assay Buffer

25 mL

-20°C

CDNB

100 μL

-20°C away from light

GSH

2 X 17 mg

-20°C

GST Positive Control (20X)

10 μL

-20°C

Shipping:  Blue ice                                 shelf life: 1 year

Features & Properties

FeaturesSimple procedure; Takes only approx. 1-1.5 hours; Fast and convenient.