Total Glutathione Detection Kit (DTNB Colorimetric Method)

Glutathione is a small peptide composed of 3 amino acid residues, the full name of which is glutamyl-cysteinyl-glycine. Because the sulfhydryl group (SH) on cysteine is the active group of glutathione, it is often abbreviated as G-SH or GSH. There are two forms of glutathione: oxidized glutathione disulfide and reduced glutathione (commonly known as GSH). Oxidized glutathione is produced by the dehydrogenation of two GSHs by sulfhydryl groups, often abbreviated as G-S-S-G or GSSG. Reduced glutathione is the main source of sulfhydryl groups in the vast majority of living cells, plays an important role in maintaining the proper redox state of sulfhydryl groups in proteins, and is a key antioxidant in animal cells. Typically, 90-95% of total glutathione is reduced glutathione.

In the detection of total glutathione, first glutathione reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH), and then GSH reacts with DTNB to produce yellow TNB, and the amount of total glutathione determines the amount of yellow TNB formation, and the amount of total glutathione can be obtained by detecting the absorbance of A₄₁₂.

This kit is a simple and easy to measure total glutathione (GSSG+GSH) for the determination of total glutathione in animal tissues, plasma, red blood cells, cultured cells, or other appropriate samples.