GSH and GSSG Assay Kit

Glutathione is a small peptide composed of three amino acid residues, with the full name of Glutamyl-cysteinyl-glycine. Since the thiol group (-SH) on cysteine is the active group of glutathione, it is often abbreviated as G-SH or GSH. Glutathione includes two forms: oxidized glutathione (Oxidized glutathione disulfide) and reduced glutathione (Reduced glutathione, commonly referred to as GSH). Oxidized glutathione is formed by the dehydrogenation of two GSH molecules through the thiol group, commonly abbreviated as G-S-S-G or GSSG. Reduced glutathione is the main source of thiol groups in the vast majority of living cells, plays an important role in maintaining the appropriate redox state of thiol groups in proteins, and is a key antioxidant in animal cells. Typically, 90-95% of the Total Glutathione is reduced glutathione.

In the detection of Total Glutathione, first, glutathione reductase reduces oxidized glutathione (GSSG) to reduced glutathione (GSH). Subsequently, GSH reacts with the chromogenic substrate DTNB to produce yellow TNB. The amount of Total Glutathione determines the formation amount of yellow TNB, and the amount of Total Glutathione can be obtained by detecting the absorbance at A₄₁₂. First, use an appropriate reagent to remove GSH from the sample, and then the content of GSSG can be determined using the above reaction principle. Subtract the content of GSSG from the total amount of glutathione (GSSG + GSH) to calculate the content of GSH.

This kit is a simple and easy-to-use detection kit that can separately detect the contents of reduced glutathione GSH and oxidized glutathione GSSG. It can be used to detect the contents of GSH and GSSG in animal tissues, plasma, red blood cells, cultured cells, or other appropriate samples. The detection limit of this kit is 0.5 μM. One kit can perform 100 detections in total, which can determine the Total Glutathione or GSSG content of 100 samples, or can determine the respective contents of GSH and GSSG in 50 samples.