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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Cell lines
PC12 cells
Preparation method
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.
Reacting condition
1–1000 μM; 3h
Applications
In PC12 cells, Acetylcysteine (NAC) produced concentration-dependent decreases in DOPAL levels. Co-incubation of NAC (1-10 μM) with selegiline (1 μM) attenuated or prevented the Cys-DA response to selegiline, without interfering with the selegiline-induced decrease in DOPAL production or inhibiting tyrosine hydroxylation.
Animal models
the R6/1 transgenic mouse model of Huntington’s disease (HD)
Dosage form
500 mg/kg i.p., 4 weeks
Application
In the R6/1 transgenic mouse model of Huntington’s disease (HD), Acetylcysteine (NAC)-HD mice showed forced-swim test (FST) immobility times intermediate to saline-HD and saline-WT mice. NAC exhibited antidepressant-like effect. HD mice co-administered with CPG (inhibitor of GLT-1) and NAC showed higher immobility times than NAC-HD mice, suggesting that the antidepressant-like effect of NAC is dependent on glutamate transport.
Other notes
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.
References:
[1]. Goldstein DS1, Jinsmaa Y2, Sullivan P2, et al. N-Acetylcysteine Prevents the Increase in Spontaneous Oxidation of Dopamine During Monoamine Oxidase Inhibition in PC12 Cells. Neurochem Res. 2017 Aug 24.
[2] Wright DJ1,2, Gray LJ3,2, Finkelstein DI1, et al. N-acetylcysteine modulates glutamatergic dysfunction and depressive behavior in Huntington's disease. Hum Mol Genet. 2016 Jul 15;25(14):2923-2933.