Luciferase (1-550, His-tag)
Luciferase (1-550, His-tag) is an enzyme expressed from a cloned gene derived from the North American firefly (Photinus pyralis). Luciferase catalyzes the luminescence of the substrate. This reaction requires the substrate luciferin, magnesium ions (Mg²⁺), and adenosine triphosphate (ATP), and produces green light with a wavelength of 562 nm.
Luciferase can be used to detect trace amounts of adenosine triphosphate (ATP). For example, using 0.2 micrograms of luciferase can detect less than or equal to one femtomole of ATP. It can also be used as a reporter gene, such as in the study of gene regulation and function, as well as in pharmaceutical screening. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression.
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Accession # |
- |
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Alternate Names |
Recombinant firefly Luciferase |
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Species |
Photinus Pyr. |
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Source |
Escherichia coli. |
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Protein sequence |
- |
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Tag |
His-tag |
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M.Wt |
Approximately 61.2 kDa |
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Appearance |
Solution protein |
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Stability & Storage |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -80°C as supplied. - 2 weeks, 2 to 8°C under sterile conditions after reconstitution. |
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Concentration |
1.0 mg/mL |
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Formulation |
Dissolved in sterile PBS buffer. |
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Reconstitution |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. This solution can be diluted into other aqueous buffers. |
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Biological Activity |
- |
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Shipping Condition |
Blue ice |
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Handling |
Centrifuge the vial prior to opening. |
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Usage |
For Research Use Only! Not to be used in humans. |
