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SUMO Protease

Catalog No.
K1103
SUMO Protease
Grouped product items
SizePriceStock Qty
200U
$60.00
In stock
1000U
$150.00
In stock
5000U
$400.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

SUMO protease (Ulp) is a recombinant cysteine protease derived from yeast Ulp1 (Ubl-specific protease 1), typically expressed in E. coli systems. It precisely recognizes and cleaves the peptide bond immediately downstream of the Gly-Gly motif at the C-terminal of SUMO (small ubiquitin-like modifier) fusion proteins. This protease is widely employed in removing SUMO fusion tags from target proteins during affinity purification, facilitating structural and functional protein analysis. The enzymatic reaction optimally occurs at temperatures around 30 ºC and pH near 8.0, yet remains active at broader ranges (2–30 ºC, pH 6.0–10.0, NaCl concentrations up to 400mM). Inclusion of 0.5–2 mM DTT in reaction significantly enhances proteolytic activity. Protease reactions performed overnight at 4 ºC are suitable for preserving unstable or temperature-sensitive targets. Post-cleavage SUMO protease with N-terminal histidine-tag can be efficiently removed via affinity chromatography.

Quality Control

Quality Control & DataSheet

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Components and Storage

Components Ingredients Specification
200 U 1000 U 5000 U
SUMO Protease (10 U/µL)

25 mM Tris-HCl, pH 8.0

0.1% Igepal (NP-40)

250 mM NaCl

500 μM DTT

50% (v/v) glycerol

20 µL 100 µL 500 µL
10X SUMO Protease Buffer + Salt

500 mM Tris acetate, pH 8.0

2% Igepal (NP-40)

1.5 M NaCl

10 mM DTT

400 µL 2 x 1 mL 10 x 1 mL
10X SUMO Protease Buffer - Salt

500 mM Tris acetate, pH 8.0

2% Igepal (NP-40)

10 mM DTT

400 µL 2 x 1 mL 10 x 1 mL

Long-term storage: -80°C