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T7 Tag Peptide Peptide sequence-MASMTGGQQMG

Catalog No.A6008
Size Price Stock Qty
5mg
$129.00
In stock
5mg
$518.00
In stock

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Sample solution is provided at 25 µL, 10mM.

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Chemical structure

T7 Tag Peptide

Biological Activity

Description T7 Tag Peptide is a 11 amino acid peptide derived from the T7 major capsid protein .
Targets anti-T7-Tag antibody          
IC50            

T7 Tag Peptide Dilution Calculator

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T7 Tag Peptide Molarity Calculator

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Chemical Properties

Cas No. SDF Download SDF
Synonyms H-Met-Ala-Ser-Met-Thr-Gly-Gly-Gln-Gln-Met-Gly-OH
Chemical Name T7 Tag
Canonical SMILES CC(C(C(=O)NCC(=O)NCC(=O)NC(CCC(=O)N)C(=O)NC(CCC(=O)N)C(=O)NC(CCSC)C(=O)NCC(=O)O)NC(=O)C(CCSC)NC(=O)C(CO)NC(=O)C(C)NC(=O)C(CCSC)N)O
Formula C41H71N13O16S3 M.Wt 1098.27
Solubility ≥54.9mg/mL in DMSO Storage Desiccate at -20°C
Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Background

The T7 tag is an epitope tag composed of an 11-residue peptide encoded from the leader sequence of the T7 bacteriophage gene10. This gene encodes a T7 major capsid protein whose function is not clear. Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein's biochemical properties.
The T7 tag is commonly engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized using immunochemical methods. The T7 tag has been used extensively as a general epitope tag in many expression vectors including the pET system that is based on T7 RNA polymerase expression systems [1].


References:
1. Studier, F.W. and Moffatt, B.A. (1986). Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. J Mol Biol 189, 113-30.