Phos binding reagent (Phosbind) Biotin
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Phosbind Biotin provides a sensitive method for detection of phosphorylated proteins on a PVDF membrane. This method needs streptavidin-conjugated horseradish (HRP) and chemiluminescent detection reagent.
Protein phosphorylation is an important covalent post-translational modification that can alter the structural conformation of a protein, which then regulates the function, location and specific binding of the target protein. Many cellular processes are regulated by the reversible phosphorylation of proteins and 30% of the proteins are likely to be phosphorylated at some point during their existence.
The determination of the phosphorylation state of proteins is important for defining protein kinase substrates and revealing the activation state of signal transduction pathways. Methods for determining the phosphorylation status of proteins are thus have important implications in the understanding of diverse biological and pathophysiological processes, such as signal transduction pathways, cancer and other diseases.
Phos binding reagents (Phosbind) are specific and selective phosphate-binding reagents, and exhibit preferential ionic interactions with phosphorylated ions on phosphorylated proteins or peptides at neutral pH. Phos binding reagent is a dinuclear metal (Zn2+ or Mn2+) complex.
Phosbind Biotin is a specific reagent for detection of phosphorylated proteins using Western Blot and can instead of the specific phosphorylated antibodies.
1. Highly sensitive detection of phosphorylated proteins
2. The binding specificity of Phos binding reagent Biotin is independent on amino acid and sequence context.
3. Followed by antibody reprobing and MS analysis are applicable.
4. The procedure is almost the same as that with an HRP-conjugated antibody.
1. M. J. Hubbard, P. Cohen. On target with a new mechanism for the regulation of protein phosphorylation. Trends Biochem. Sci. 18: 172 (1993).
2. B. Agnew et al. Compositions and methods for detection and isolation of phosphorylated molecules. US Patent # 7,102,005. (September 5, 2006).
|Solubility||insoluble in H2O; insoluble in EtOH; ≥32.3 mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
Quality Control & MSDS
- View current batch:
Purity = 98.00%
- MSDS (Material Safety Data Sheet)