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HyperScribe™ T7 High Yield RNA Synthesis Kit

In stock
Catalog No.
K1047
For high-efficiency in vitro transcription
Grouped product items
SizePriceStock Qty
25 rxns
$100.00
In stock
50 rxns
$180.00
In stock
100 rxns
$350.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

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Components and Storage

Components 25 rxns 50 rxns 100 rxns
T7 RNA Polymerase Mix 50 ul 100 ul 200 ul
10 × Reaction Buffer 50 ul 100 ul 200 ul
ATP (20 mM) 50 ul 100 ul 200 ul
GTP (20 mM) 50 ul 100 ul 200 ul
UTP (20 mM) 50 ul 100 ul 200 ul
CTP (20 mM) 50 ul 100 ul 200 ul
Control Template(0.5 ug/ul) 5 ul 10 ul 20 ul
RNase-free H2O 0.5 ml 1 ml 2×1 ml

Store all the kit components at -20°C.

Description

HyperScribe™ T7 High Yield RNA Synthesis Kit performs in vitro RNA transcription experiment in a relatively short time using T7 RNA polymerase. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides.

RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.

The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 μl each time. Up to 180 μg of RNA can be generated with 1 μg of control template per standard reaction. Each 25/50/100 reactions kit can produce up to 4.5 mg/9 mg/18 mg RNA.

Figure 1: Transcription using T7 RNA Polymerase

Figure 2: PCR primer design for T7 RNA Polymerase template