HyperScribe™ T7 High Yield RNA Synthesis Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Quality Control & DataSheet
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Components and Storage
|Components||25 rxns||50 rxns||100 rxns|
|T7 RNA Polymerase Mix||50 ul||100 ul||200 ul|
|10 × Reaction Buffer||50 ul||100 ul||200 ul|
|ATP (20 mM)||50 ul||100 ul||200 ul|
|GTP (20 mM)||50 ul||100 ul||200 ul|
|UTP (20 mM)||50 ul||100 ul||200 ul|
|CTP (20 mM)||50 ul||100 ul||200 ul|
|Control Template(0.5 ug/ul)||5 ul||10 ul||20 ul|
|RNase-free H2O||0.5 ml||1 ml||2×1 ml|
Store all the kit components at -20°C.
HyperScribe™ T7 High Yield RNA Synthesis Kit performs in vitro RNA transcription experiment in a relatively short time using T7 RNA polymerase. The kit is designed for high yields of RNA transcripts, highly specific radiolabeled RNA probes and to obtain capped, dye-labeled or biotinylated RNA incorporated modified nucleotides.
RNA synthesized using this kit covers a wide range of applications, such as in vitro translation, antisense RNA and RNAi experiments, RNA vaccines, RNA structure and function studies, ribozyme biochemistry, RNase protein experiments and probe-based hybridization blots.
The kit contains sufficient reagents to carry out 25/50/100 reactions, 20 μl each time. Up to 180 μg of RNA can be generated with 1 μg of control template per standard reaction. Each 25/50/100 reactions kit can produce up to 4.5 mg/9 mg/18 mg RNA.
Figure 1: Transcription using T7 RNA Polymerase
Figure 2: PCR primer design for T7 RNA Polymerase template