Emodin

mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail

Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.

Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody

Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay

SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.

Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Storage | Store at -20°C |
M.Wt | 270.24 |
Cas No. | 518-82-1 |
Formula | C15H10O5 |
Synonyms | Emodol;Frangula emodin |
Solubility | ≥27 mg/mL in DMSO,insoluble in EtOH,insoluble in H2O |
Chemical Name | 1,3,8-trihydroxy-6-methylanthracene-9,10-dione |
SDF | Download SDF |
Canonical SMILES | CC1=CC(O)=C2C(C(C3=CC(O)=CC(O)=C3C2=O)=O)=C1 |
Shipping Condition | Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request. |
General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
Cell experiment [1]: | |
Cell lines |
Epithelial ovarian cancer (EOC) cells |
Preparation method |
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
Reacting condition |
0 ~ 80 μM; 48 hrs |
Applications |
In a series of EOC cells, Emodin inhibited cell proliferation in a dose-dependent manner. Emodin at the dose of 20 μM abrogated the migration and invasion abilities of A2780 and SK-OV-3 cells transfected with pLVX-ILK. The results of western blotting analysis implied that Emodin inhibited the migration and invasion abilities of EOC cells by blocking epithelial-mesenchymal transition (EMT) through targeting integrin-linked kinase (ILK). |
Animal experiment [1]: | |
Animal models |
Nude mice bearing SK-OV-3/pLVX-ILK cells |
Dosage form |
50 mg/kg/d; i.p.; for 21 days |
Applications |
In nude mice bearing SK-OV-3/pLVX-ILK cells, Emodin significantly inhibited tumor growth. Meanwhile, Emodin counteracted the effects of over-expression of ILK on EOC cells. The immunohistochemical results demonstrated that Emodin inhibited the EMT of tumor cells. In addition, Emodin exhibited mild cardiac, liver and renal toxicities in vivo. |
Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
References: [1]. Lu J, Xu Y, Zhao Z, Ke X, Wei X, Kang J, Zong X, Mao H, Liu P. Emodin suppresses proliferation, migration and invasion in ovarian cancer cells by down regulating ILK in vitro and in vivo. Onco Targets Ther. 2017 Jul 19;10:3579-3589. |
Quality Control & MSDS
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Chemical structure
