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Cy5.5 azide (non-sulfonated)

Catalog No.
A8114
used for Click chemistry to label alkyne-modified oligonucleotides
Grouped product items
SizePriceStock Qty
5mg
$460.00
In stock
25mg
$1,350.00
In stock

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Background

This Cy5.5 is a labeling reagent that ready for the use in Click Chemistry reaction which available as 10 mM solution in DMSO. As Cy5.5 azide is a far-red emitting dye, it can replace Alexa Fluor 680, and DyLight 680. Most derivatives of non-sulfonated cyanines have low aqueous solubility except for hydrochlorides of hydrazides and amines. For biomolecule labeling, the labeling reagent has low aqueous solubility, using of organic co-solvent to dissolve this molecular is necessary for efficient reaction. First, Cyanine dye should be dissolved in organic solvent and then added to a solution of biomolecule in appropriate aqueous buffer.

In human glioblastoma U87MG cells, to assess the capability of the Cu(I)/M-dots for cell labeling Cy5.5-azide was in combination with Cu(I)/M-dots for imaging of the integrin αⅤβ3 receptor [1].

Reference:
[1] Sun, Y. ; Hong, S.; Ma, X.; Cheng, K.; Wang, J.; Zhang, Z.; Yang, M.; Jiang, Y.; Hong, X.; Cheng, Z. Recyclable Cu(I)/Melanin Dots for Cycloadditions, Bioconjugation and Cell Labeling. Chemical Science.

Chemical Properties

Physical AppearanceA solid
Storage24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks. Avoid prolonged exposure to light. Desiccate.
M.Wt701.34
FormulaC43H49ClN6O
Solubility≥70.1mg/mL in DMSO, ≥46.2 mg/mL in EtOH with ultrasonic,insoluble in H2O
Chemical Name3-(6-((3-azidopropyl)amino)-6-oxohexyl)-1,1-dimethyl-2-((1E,3E,5E)-5-(1,1,3-trimethyl-1H-benzo[e]indol-2(3H)-ylidene)penta-1,3-dien-1-yl)-1H-benzo[e]indol-3-ium chloride
SDFDownload SDF
Canonical SMILESCC1(C)C2=C3C(C=CC=C3)=CC=C2[N+](CCCCCC(NCCCN=[N+]=[N-])=O)=C1/C=C/C=C/C=C4C(C)(C)C5=C(C=CC=C6)C6=CC=C5N\4C.[Cl-]
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Spectral properties

Excitation max (nm) 684 Emission max (nm) 710
Extinction Coefficient (M-1cm-1) 209000 Quantum Yield 0.2
CF260 0.07 CF280 0.03

Quality Control

Chemical structure

Cy5.5 azide

Related Biological Data

Cy5.5 azide