BIBO 3304 trifluoroacetate
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
BIBO 3304 is a high affinity NPY Y1 receptor antagonist with IC50 values of 0.72 and 0.38 nM at rat and human receptors respectively .
Neuropeptide Y is a 36 amino acid polypeptide that is expressed in the hypothalamus. It is an endocrine and neuronal messenger involved in many physiological processes such as elevates blood pressure and stimulates food intake .
BIBO 3304 displayed high affinity (IC50=0.69+0.16 nM) for the human Y1 receptor stably expressed in BHK cells and a higher affinity(IC50=0.38+0.06 nM) for SK-N-MC cells, a human neuroblastoma cell line endogenously expressing the Y1 receptor. BIBO 3304 exhibited selective binding to the Y1 receptor subtype and more than 1000 ± 10,000-fold lower affinity for the human Y2 receptor, the human and rat Y4 receptor as well as the human and rat Y5 receptor. In SK-N-MC cells, the NPY induced inhibition of cAMP synthesis was antagonized by 100 nM BIBO 3304 with a pKb of 9.1+0.4 .
In a rat model, BIBO 3304 inhibited feeding response mediated by 1mg NPY in a dose-dependent way. A dose of 30mg caused an approximately 50% inhibition (1.87+0.3 g, n=18). BIBO 3304 (30mg) had no effect on noradrenaline or galanin induced feeding. However, it can block the feeding response mediated by NPY (2 ± 36) (1mg), NPY (3 ± 36) (1mg) and [Leu31, Pro34]NPY (2mg) . BIBO 3304 also antagonizes anxiolytic-like effects of NPY in the basolateral nucleus of the amygdala in rats .
. Wieland HA, Engel W, Eberlein W, et al. Subtype selectivity of the novel nonpeptide neuropeptide Y Y1 receptor antagonist BIBO 3304 and its effect on feeding in rodents. Br J Pharmacol, 1998, 125(3): 549-55.
. Sajdyk TJ, Vandergriff MG, Gehlert DR. Amygdalar neuropeptide Y Y1 receptors mediate the anxiolytic-like actions of neuropeptide Y in the social interaction test. Eur J Pharmacol, 1999, 5;368(2-3): 143-147.
|Physical Appearance||White solid|
|Storage||Store at -20°C|
|Solubility||<75.77mg/ml in DMSO; <75.77mg/ml in ethanol|
|Chemical Name||2,2,2-trifluoroacetic acid compound with (R,Z)-5-guanidino-N-(4-(((hydroxy(imino)methyl)amino)methyl)benzyl)-2-((Z)-(1-hydroxy-2,2-diphenylethylidene)amino)pentanimidic acid (2:1)|
|Canonical SMILES||N=C(NCCC[[email protected]](/N=C(O)/C(C1=CC=CC=C1)C2=CC=CC=C2)([H])/C(O)=N/CC3=CC=C(CNC(O)=N)C=C3)N.FC(F)(F)C(O)=O.FC(F)(F)C(O)=O|
|Shipping Condition||Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request|
|General tips||For obtaining a higher solubility , please warm the tube at 37°C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.|