Ac-Endothelin-1 (16-21), human
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
ENDOTHELIN-1 (ET-1), the principal peptide of the endothelin family, has been shown to have a variety of biological activities in both vascular and nonvascular tissues, including the heart, the kidney, and the central nervous system.
At the cellular level, the effects of ET-1 appear to be mediated by two speciﬁc receptor subtypes termed ETA and ETB, both of which are functionally coupled to phospholipase C (PLC).
The C-terminal fragment , which is a highly conserved sequence in the peptides of the ET family, has been shown to discriminate between the two ET receptor subtypes termed ETA and ETB since it activates only the latter ones.
1. Rubanyi, G. M., and M. A. Polokoff. Endothelins: molecular biology, biochemistry, pharmacology, physiology, and pathophysiology. Pharmacol. Rev. 46: 325–415, 1994
2. Simonson MS. Endothelins: multifunctional renal peptides. Physiol Rev 1993; 73: 375–411.
3. PM Vanhoutte Endothelin-1. A matter of life and breath, Nature, 368 (1994), pp. 693–694
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥83.8mg/mL in DMSO with gentle warming|
|Canonical SMILES||O=C(N[[email protected]@H](CC(C)C)C(N[[email protected]@H](CC(O)=O)C(N[[email protected]@H]([[email protected]](C)CC)C(N[[email protected]@H]([[email protected]](C)CC)C(N[[email protected]@H](CC1=CC(C=CC=C2)=C2N1)C(O)=O)=O)=O)=O)=O)[[email protected]](CC3=CNC=N3)NC(C)=O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|