In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Windorphen is a Wnt inhibitor that selectively abrogates the Wnt signaling for ventral development.
Wnt protein is a family of signaling proteins that interacts on the Wnt/β-Catenin pathways during embryotic development, cell proliferation, migration and adult tissue homeostasis.
In canonical Wnt/β-catenin reporter cell line STF293, Windorphen dose-dependently inhibits Wnt3a-inducible TOPFLASH-luciferase activity (IC50 of 1.5 mM). In human colon adenocarcinoma
SW480 cells with defective APC gene that constitutively activates Wnt signaling, 72 h treatment of Windorphen causes a wide spread apoptosis. 
In zebrafish embryos, Windorphen leads to apparent expansion of the dorsal markers (pax2.1) and marker of rhombomeres 3 and 5 at the six-somite stage (krox0). Windorphen selectively inhibits Wnt signaling in ventral and lateral regions of the 50% epiboly stage embryo (5.3 hpf). In addition, Windoprhen treatment rescues the telencephalon/eye phenotype in the mbl mutant zebrafish (i.e. a defective Axin1/β-catenin destruction complex causes abnormal activation of Wnt signaling and the loss of telencephalon and eyes.) 
1. Hao J, Ao A, Zhou L et al. Selective small molecule targeting β-catenin function discovered by in
vivo chemical genetic screen. Cell Rep. 2013 Sep 12;4(5):898-904.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||insoluble in EtOH; insoluble in H2O; ≥11.45 mg/mL in DMSO|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.|