In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
NSC 319726 is a reactivator of mutant p53 with IC50 value of 8nM .
NSC 319726 belongs to the thiosemicarbazone family. In a mouse fibroblast cell line Balb/c 3T3, NSC 319726 shows greater inhibition of cell growth in cells expressing mutant p53 than in WT p53 control. NSC 319726 is also nontoxic to WI38 human fibroblasts (p53 WT). In three different ovarian carcinoma cell lines (TOV112D (p53R175H), OVCAR3 (p53R248W) and SKOV3 (p53-/-)), treatment of NSC 319726 results in an induction of apoptosis in the p53R175 mutant. NSC 319726 restores p53 function through inducing a conformation change of mutant p53 to a “WT-like” structure which can be recognized by the WT specific antibody. The inhibition of cell growth caused by NSC 319726 is also found in the TOV112D (p53R175H) xenografts when the dose of NSC 319726 is 1mg/kg. Furthermore, it is shown that zinc ion chelation and ROS changes are important for the apoptosis induced by NSC319726 .
 Yu X, Vazquez A, Levine AJ, Carpizo DR. Allele-specific p53 mutant reactivation. Cancer Cell. 2012 May 15;21(5):614-25.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥8.1 mg/mL in DMSO, ≥3.43 mg/mL in EtOH with ultrasonic and warming,insoluble in H2O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
|Description||NSC 319726 is a reactivator of mutant p53 with IC50 value of 8 nM for fibroblasts expressing the p53R175 mutation.|
|IC50||8 nM (in fibroblasts expressing the p53R175 mutation)|