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In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Store all the kit components at -20°C.
HyperScribe™ T7 High Yield Fluorescein RNA Labeling Kit is designed to generate randomly fluorescein-modified RNA probes by in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern blot hybridization experiments. The principle of labeling is similar to the basic labeling principle of a mixture of fluorescein RNA labels. Fluorescein-12-UTP is efficiently incorporated into RNA using the optimized reaction buffer and T7 RNA polymerase mixture in place of its natural counterpart UTP. An appropriate Fluorescein-12-UTP substitution typically achieves an optimal balance between reaction and labeling efficiency. However, the individual optimization of the Fluorescein-12-UTP / UTP ratio can be easily achieved using a single nucleotide format. The resulting fluorescein-modified RNA probe can then be detected by fluorescence spectroscopy.
