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Glutathione Colorimetric Assay Kit
Detects either total glutathione or exclusively GSH

Catalog No.K2106
Size Price Stock Qty
100 assays
$414.00
In stock

Tel: +1-832-696-8203

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Sample solution is provided at 25 µL, 10mM.

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Quality Control & DataSheet

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Overview

Kit ComponentsCatalog NoK2106
Glutathione Reaction Buffer uffer
Glutathione Substrate (DTNB)
NADPH Generating Mix (lyophilized) Glutathione Reductase (lyophilized)
Sulfosalicylic Acid (SSA, 1 gram)
GSH Standard (lyophilized, MW 307)
Product nameGlutathione Colorimetric Assay Kit
Detection methodAbsorbance (412 nm)
Sample typeCell and Tissue lysates, culture media, urine, plasma and serum, as well as many other biological fluids.
Species reactivityMammalian
Applications: The Colorimetric Glutathione Detection Kit provides a simple in vitro assay for detecting the GSH changes in apoptosis and other pathological processes.

Features & Properties

FeaturesSimple procedure; takes only ~2-3 hours
Fast and convenient
ShippingGel pack
Storage ConditionsStore at -20°C.
UsageFor Research Use Only! Not For Use in Humans.

Description

Glutathione (GSH) is a low-molecular-weight thiol that play a vital role in protecting cell from oxidative stress in mammalian cells. The Glutathione Colorimetric Assay Kit provides an easy and colorimetic method for analyzing totoal glutathione or the reduced from of glutathione by a microtiter plate reader. The assay utilizes glutathione recycling system by DTNB and glutathione reductase. The yellow color 2-nitro-5-thiobenzoic acid that generate by the reaction of DTNB and GSH. Thus the GSH concentration can be measured at 412 nm absorbance. The generated GSSG can be reduced back to GSH by glutathione reductase, and GSH reacts with DTNB again to produce more 2-nitro-5-thiobenzoic acid. As a result, the recycling system significantly improves the sensitivity of total GSH detection. The 5-Sulfosalicylic acid (SSA) in the kit removes proteins from samples to protect the GSH oxidation and γ-glutamyl transpeptidase reaction. The kit can quantify glutathione from 1-100 ng/well in a 200 μl reaction. Increase the reaction time to generate stronger signal for detecting lower glutathione concentrations, such as in blood samples. The kit can also specifically detect the reduced form of glutathione (GSH) by omitting the glutathione reductase from the reaction mixture. The sensitivity for detecting the reduced form of glutathione (without recycling system) is 100 times lower than detecting the total glutahione.