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Caspase-1 Colorimetric Assay Kit

Catalog No.
K2011
Detects YVAD-dependent caspase-1 activity, highly-sensitive
Grouped product items
SizePriceStock Qty
25 assays
$179.00
Ship with 5-10 days
100 assays
$403.00
Ship with 5-10 days
200 assays
$615.00
Ship with 5-10 days
400 assays
$986.00
Ship with 5-10 days
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-1, also known as IL-1β-converting enzyme (ICE), is a key regulator of innate immunity. The active Caspase-1 cleaves and activates pro-inflammatory IL-1β and IL-18, and promotes an inflammatory form of cell death induced by apoptosis and pyroptosis.

Caspase-1 Colorimetric Assay Kit provides a convenient and simple way for detecting the YVAD-dependent caspase activity. When cleavage of YVAD from the labeled substrate YVAD-p-nitroaniline (YVAD-pNA) by Caspase-1 or related caspases, the free pNA light emission can be quantified by using a microplate reader or a spectrophotometer at 405 nm or 400 nm. Comparison of the absorbance of free pNA from an apoptotic sample with an uninduced control determines the fold increase in Caspase-1 activity.

Features

Simple one-step procedure; takes 1-2 hours

Fast and convenient

Comparison of the absorbance of pNA from a treated sample with an untreated control allows determination of the fold increase in Caspase-1 activity.

Quality Control

Quality Control & DataSheet

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Components and Storage

Components

K2011-25

25 Assays

K2011-100

100 Assays

K2011-200

200 Assays

K2011-400

400 Assays

Cell Lysis Buffer 25 mL 100 mL 100 mL 100 mL
2X Reaction Buffer 2 mL 4X 2 mL 16 mL 32 mL
YVAD- pNA (4 mM) 125 µL 500 µL 2X 0.5 mL 2X 1 mL
DTT (1 M) 100 µL 400 µL 400 µL 400 µL

Store the components at -20°C.