Caspase-4 Colorimetric Assay Kit
Cysteine-containing aspartate proteolytic enzymes (Caspase) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Caspase-1/4/5/11 are very important in inflammatory response and pyroptosis. Caspase-4/5 can recognize and bind intracellular lipopolysaccharide (LPS), which subsequently activate inflammasomes, which in turn promote the maturation and release of inflammatory mediator IL-1β, leading to an inflammatory response. In addition, activated caspase-4/5 can also cleave the substrate protein GSDMD, triggering the formation of cell membrane pores, resulting in the release of cell contents, and ultimately pyroptosis.
Caspase-4 Colorimetric Assay Kit provides a convenient and simple method for the detection of LEVD-dependent caspase activity. When Caspase-4 or related caspases cleave LEVD from the labeled substrate LEVD-p-nitroaniline (LEVD-pNA), the light emission of free pNA can be measured at 405 nm or 400 nm using a microplate reader or spectrophotometer. The absorbance of free pNA in apoptotic samples was compared to an uninduced control to determine a fold increase in Caspase-4 activity.
Figure 1: Reference standard curve for this kit
| Components |
K2199-25T |
K2199-50T |
K2199-100T |
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Reagent I |
20 mL |
20 mL |
25 mL |
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Reagent II |
30 mL |
60 mL |
120 mL |
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Reagent III |
0.25 mL |
0.55 mL |
2 X 0.55 mL |
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pNA Standard (5 mM) |
1 mL |
1 mL |
1 mL |
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Store the kit at -20°C, stable for 6 months. Reagent III and pNA Standard (5 mM) should be stored away from light. |
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| Features |
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