Caspase-2 Colorimetric Assay Kit
Cysteine-containing aspartate proteolytic enzymes (Caspase) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Caspase-2 is considered to be an upstream initiator of apoptosis signaling, and activation of caspase-2 can trigger a series of downstream events, including activation of executor caspases (e.g., caspase-3, caspase-7), thereby promoting the execution of apoptosis.
Caspase-2 Colorimetric Assay Kit provides a convenient and simple method for the detection of VDVAD-dependent caspase activity. When caspase-2 or related caspases cleave VDVAD from the labeled substrate VDVAD-p-nitroaniline (VDVAD-pNA), the light emission of free pNA can be measured at 405 nm or 400 nm using a microplate reader or spectrophotometer. The absorbance of free pNA in apoptotic samples was compared to an uninduced control to determine a fold increase in caspase-2 activity.
Figure 1: Reference standard curve for this kit
| Components |
K2017-25T |
K2017-50T |
K2017-100T |
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Reagent I |
20 mL |
20 mL |
25 mL |
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Reagent II |
30 mL |
60 mL |
120 mL |
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Reagent III |
0.25 mL |
0.55 mL |
2 X 0.55 mL |
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pNA Standard (5 mM) |
1 mL |
1 mL |
1 mL |
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Store the kit at -20°C, stable for 6 months. Reagent III and pNA Standard (5 mM) should be stored away from light. |
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| Features |
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