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Genistein

In stock
Catalog No.
A2198
ER agonist
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$55.00
In stock
100mg
$50.00
In stock

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Background

Phytoestrogen with a wide range of biological actions. Inhibits protein tyrosine kinases including epidermal growth factor receptor kinase. Also binds to PPARγ and estrogen receptors and acts as an agonist at GPR30. Also available as part of the MAP

Chemical Properties

StorageStore at -20°C
M.Wt270.24
Cas No.446-72-0
FormulaC15H10O5
Solubility≥55.6mg/mL in DMSO
Chemical Name5,7-dihydroxy-3-(4-hydroxyphenyl)chromen-4-one
SDFDownload SDF
Canonical SMILESC1=CC(=CC=C1C2=COC3=CC(=CC(=C3C2=O)O)O)O
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.

Protocol

Cell experiment [1]:

Cell lines

NIH-3T3 cells

Preparation method

The solubility of this compound in DMSO is > 55.6 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

0 ~ 1000 μM

Applications

NIH-3T3 cell growth was inhibited after Genistein treatment at concentrations of 4 ~ 75 μM for 72 hrs. At concentrations below 40 μM, Genistein-treated cells recovered growth after drug withdrawal. In contrast, at 75 μM or above, Genistein-treated cells were unable to recover growth. Cytotoxicity assessed by cloning efficiency measured after 3 hrs of Genistein treatment showed an ED50 value of 35 μM.

Animal experiment [2]:

Animal models

Female SD rats

Dosage form

0, 25 and 250 mg Genistein/kg AIN-76A; p.o.

Applications

In female SD rats, Genistein dose-dependently suppressed dimethylbenz[a]anthracene (DMBA)-induced mammary tumor development. Rats exposed to 25 and 250 mg Genistein/kg AIN-76A showed 7.1 and 4.3 mammary tumors, respectively. Dietary Genistein protected against mammary tumors through regulating specific sex steroid receptors as well as growth factor signaling pathways.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Linassier C, Pierre M, Le Pecq JB, Pierre J. Mechanisms of action in NIH-3T3 cells of genistein, an inhibitor of EGF receptor tyrosine kinase activity. Biochem Pharmacol. 1990 Jan 1;39(1):187-93.

[2]. Lamartiniere CA, Cotroneo MS, Fritz WA, Wang J, Mentor-Marcel R, Elgavish A. Genistein chemoprevention: timing and mechanisms of action in murine mammary and prostate. J Nutr. 2002 Mar;132(3):552S-558S.

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