EZ Cap™ Firefly Luciferase mRNA (5-moUTP)
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
EZ Cap™ Firefly Luciferase mRNA (5-moUTP) will express luciferase protein once entering cells, which is initially extracted from firefly Photinus pyralis. This enzyme catalyzes ATP-dependent D-luciferin oxidation and lead to yield chemiluminescence at about 560 nm wavelength. Firefly Luciferase is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc.
EZ Cap™ Firefly Luciferase mRNA (5-moUTP) is provided at a concentration of 1mg/ml. It is co-transcriptional capped by EZ Cap™ Reagent AG (3' OMe) (Catalog No.B8178) which generates a cap 1 structure with high efficiency. Cap 1 structure is more ideal for mammalian systems and possesses higher transcription efficiency than Cap 0 structure (ARCA and mCap). EZ Cap™ Reagent AG (3' OMe) obtain the ability only inserting in the proper orientation, results in forming mRNAs that can be translated twice efficiently as those initiated with EZ Cap™ Reagent AG. The addition of 5-moUTP and poly (A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
All the modifications are intention to mimics a fully processed mature mRNA. EZ Cap™ Firefly Luciferase mRNA (5-moUTP) is an ideal product to observing mRNA delivery, translation and other behaviors.
Related Biological Data
| mRNA Length | 1921 nucleotides | ||
| Concentration | 1 mg/mL | ||
| Buffer | 1 mM Sodium Citrate, pH 6.4 | Storage | -40°C or below |
| General tips | Please dissolve it on ice and protect from RNase carefully. Avoid repeated freeze/thaw cycles as possible. Don’t vortex. Upon first use, centrifuge the tube softly and aliquot it into several single use portions. Use RNase-free reagents and materials with appropriate RNase-free technique. Don’t add to the media with serum unless mixing with a transfection reagent. | ||
| Shipping Condition | Evaluation sample solution: ship with dry ice. | ||
