3T3-msCD40L Cell Lines
3T3-msCD40L (40LB) cells are a murine BALB/c 3T3-derived feeder cell line stably engineered to co-express mouse CD40 ligand (CD40L) and B-cell activating factor (BAFF). These cells are functionally validated to provide essential activation and survival signals for naive mouse B cells in vitro, serving as the core supporting component of the established iGB (induced Germinal Center B cell) culture system.
Following irradiation to arrest proliferation while preserving ligand bioactivity, 3T3-msCD40L cells drive robust B cell expansion and efficient class-switch recombination, effectively recapitulating the germinal center reaction in a controlled in vitro environment. This system provides a reliable platform for the mechanistic dissection of B cell differentiation, affinity maturation, and humoral immune responses.
Reference:
Haniuda, K. and Kitamura, D. (2019). Induced Germinal Center B Cell Culture System. Bio-protocol 9(4): e3163.
| Alternative name | 40LB, 3T3/40L/BAFF |
| Cell Type | Mouse BALB/c 3T3-derived feeder fibroblast cell line stably co-expressing mouse CD40L and BAFF |
| Accession ID | CVCL_A6ZS |
| Organism | Mouse |
| Subculturing | Passage every 3 days at 1:6 dilution in 10 cm dish with 10 mL 3T3 medium |
| Maintenance Tips | Irradiate before co-culture to arrest proliferation while preserving CD40L/BAFF bioactivity. Plate 0.5×10⁶ cells per well in 6-well plate; allow cells to attach before use. |
| Propagation Medium | High-glucose DMEM + 10% heat-inactivated FBS + 100 U/mL penicillin + 100 μg/mL streptomycin |
| Storage | -150°C~-196°C (liquid-nitrogen). |
| Shipping Condition | Ship with dry ice (-80°C). |
| Note | For laboratory research only. Not for clinical applications. |
