3T3-msCD40L Cell Lines
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
3T3-CD40L is a mouse fibroblast cell line that secretes mouse CD40L. 3T3-CD40L cells can be co-cultured with B cells to aid B cell expansion .
 J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013).
Quality Control & MSDS
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|Alternative name||3T3-msCD40L, 3T3-CD40L|
|Cell Type||Mouse 3T3 cell line expression CD40L|
|Subculturing||Adherent Cells, like fibroblast cells|
|Maintenance Tips||3T3-CD40L cells should be irradiated in advance so that the cells secrete mouse CD40L for B cell activation and die off. Please see the reference for more details.|
|Propagation Medium||DMEM with 10% FBS, 1% L-glutamine, and 0.1% gentamicin.|
|Note||For laboratory research only. Not for clinical applications.|