Human B Cell Culture and Expansion Kit
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Human B cell culture and expansion kit is for activation and expansion of human B cells. Human monoclonal antibodies isolated for specific antibodies are widely used in immunity studies, historical studies and therapeutic development. Memory B cells derived from peripheral blood will produce high concentration of IgG in the supernatant under the stimulation of CD40L with other growth factors . The B Cell Expansion Kit contains special treated feeder cells, expressing CD40L in activated form as well as human cell factors A and B potently inducing antibody-secret. The monoclonal antibody screening can be completed within 2 weeks with the help of the kit.
 J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013).
Quality Control & MSDS
- View current batch:
|Alternative name||B Cell Expansion Kit|
|Components||Special treated feeder cells, 7 x 106|
Human cell factor A solution, premium grade
Human cell factor B solution, premium grade
|Note||For laboratory research only. Not for clinical applications.|