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Human B Cell Culture and Expansion Kit

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1 package
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Human B cell culture and expansion kit is for activation and expansion of human B cells.  Human monoclonal antibodies isolated for specific antibodies are widely used in immunity studies, historical studies and therapeutic development. Memory B cells derived from peripheral blood will produce high concentration of IgG in the supernatant under the stimulation of CD40L with other growth factors [1]. The B Cell Expansion Kit contains special treated feeder cells, expressing CD40L in activated form as well as human cell factors A and B potently inducing antibody-secret. The monoclonal antibody screening can be completed within 2 weeks with the help of the kit.


[1] J. Huang, N. A. Doria-Rose, N. S. Longo, L. Laub, C.-L. Lin, E. Turk, B. H. Kang, S. A. Migueles, R. T. Bailer, J. R. Mascola, M. Connors, Isolation of Human Monoclonal Antibodies from Peripheral Blood B Cells. Nat. Protoc. 8, 1907-1915 (2013).

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Alternative nameB Cell Expansion Kit
ComponentsSpecial treated feeder cells, 7 x 106
Human cell factor A solution, premium grade
Human cell factor B solution, premium grade
Biosafety Level2
NoteFor laboratory research only. Not for clinical applications.