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Streptozotocin

Catalog No.
A4457
Nitrosourea compound; for type 1 diabetes animal models
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$66.00
In stock
100mg
$55.00
In stock
200mg
$77.00
In stock
500mg
$98.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

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Email: [email protected]

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Background

Streptozotocin (CAS 18883-66-4) is a naturally occurring nitrosourea antibiotic, widely recognized for its utility in biomedical research. Functioning primarily as a DNA-alkylating agent, streptozotocin preferentially targets pancreatic β-cells due to its uptake via the glucose transporter GLUT2, leading to selective cytotoxicity. This mechanism facilitates the induction of β-cell apoptosis by causing DNA damage, resulting in the destruction of insulin-producing cells.

In experimental settings, streptozotocin is used to induce hyperglycemia and diabetes mellitus in various animal models, particularly rodents, through a single or multiple dosing regimen. The cytotoxic effect of streptozotocin on pancreatic β-cells has been quantitatively reported, with specific IC50 values determined in isolated islet cells or relevant pancreatic cell lines. Additionally, streptozotocin exhibits alkylating activity that disrupts cellular metabolism and may influence other tissues expressing the GLUT2 transporter, although its primary action centers on pancreatic islet cells.

In the context of pharmaceutical and biomedical research, streptozotocin is widely employed for the experimental induction of diabetes to study the pathophysiology of the disease and to evaluate novel therapeutic agents aimed at glycemic control, β-cell protection, or diabetes-related complications.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt265.22
Cas No.18883-66-4
FormulaC8H15N3O7
SynonymsSTZ; NSC-85998; Streptozocin; U 9889
Solubility≥10.3 mg/mL in DMSO; ≥26.5 mg/mL in EtOH with gentle warming; ≥53.2 mg/mL in H2O
Chemical Name1-methyl-1-nitroso-3-[2,4,5-trihydroxy-6-(hydroxymethyl)oxan-3-yl]urea
SDFDownload SDF
Canonical SMILESCN(C(N[C@H]([C@H]1O)[C@H](O)O[C@H](CO)[C@H]1O)=O)N=O
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment:[1]

Cell lines

A murine pancreatic β cell line, INS-1

Reaction Conditions

15 or 30 mM streptozocin for 1 h incubation

Applications

Higher rates of apoptosis, as compared to necrosis, were observed when cells were exposed to 15 mM streptozocin for 1 h followed by a 24 h recovery period. Higher doses of streptozocin (30 mM) caused the cells to undergo necrosis (22%) as well as apoptosis (17%). Streptozotocin at low doses induced apoptosis and at high doses caused necrosis in INS-1 cells.

Animal experiment:[2]

Animal models

Male adult Holtzman rats

Dosage form

50, 65 or 100 mg/kg

A single intravenous injection

Applications

Streptozotocin (65 mg/kg) resulted in rapid degranulation of β cells, accumulation of glycogen in the proximal convoluted tubules of the kidney, as well as development of cataracts in 4 months after streptozotocin treatment. At a higher dose of 100 mg/kg, streptozotocin produced lesions in the exocrine cells of the pancreas, and led to persistence of small, possibly secretory, granules in the Golgi zone of β cells in diabetic rats. Streptozotocin is often used to induce diabetes mellitus in experimental animals.

Note

The technical data provided above is for reference only.

References:

1. Saini KS, Thompson C, Winterford CM, et al. Streptozotocin at low doses induces apoptosis and at high doses causes necrosis in a murine pancreatic beta cell line, INS-1. Biochemistry and Molecular Biology International, 1996, 39(6): 1229-1236.

2. Arison RN, Ciaccio EI, Glitzer MS, et al. Light and electron microscopy of lesions in rats rendered diabetic with streptozotocin. Diabetes, 1967, 16(1): 51-56.

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