Toggle Nav

Resiquimod (R-848)

Catalog No.
B1054
Immune response modifier
Grouped product items
SizePriceStock Qty
10mM (in 1mL DMSO)
$66.00
In stock
10mg
$60.00
In stock
20mg
$70.00
In stock
25mg
$120.00
In stock
50mg
$150.00
In stock
100mg
$330.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Background

Resiquimod (R-848, S-27609) is a modifier of immune response [1].

Resiquimod (R-848, S-27609) is the analogue of imiquimod (R-837,S-26308). Resiquimod has been reported to dose-dependently induce some cytokines including IFN, TNF, IL-1β and IL-6 in human peripheral blood mononuclear (PBMCs). In addition, Resiquimod has been revealed to stimulate intracellular IL-1βincreased approximately 15%. The results have also been noted that both monocytes and B cells produced IFN in response to Resiquimod. Besides, Resiquimod has determined the induced increases in IFN-α , TNF, and IL-8 mRNA expression in PBMCs when compared with the untreated cell. Moreover, Resiquimod has been found to be an effective antiviral and antitumor agent in animal models [1].

References:
[1] Gibson SJ1, Imbertson LM, Wagner TL, Testerman TL, Reiter MJ, Miller RL, Tomai MA.Cellular requirements for cytokine production in response to the immunomodulators imiquimod and S-27609. J Interferon Cytokine Res. 1995 Jun; 15(6):537-45.

Product Citation

Chemical Properties

StorageStore at -20°C
M.Wt314.38
Cas No.144875-48-9
FormulaC17H22N4O2
Solubility≥33.8 mg/mL in EtOH with ultrasonic, ≥1.62 mg/mL in H2O with ultrasonic and warming
Chemical Name1-[4-amino-2-(ethoxymethyl)imidazo[4,5-c]quinolin-1-yl]-2-methylpropan-2-ol
SDFDownload SDF
Canonical SMILESCCOCC1=NC2=C(N1CC(C)(C)O)C3=CC=CC=C3N=C2N
Shipping ConditionEvaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tipsFor obtaining a higher solubility , please warm the tube at 37°C and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20°C for several months.

Protocol

Cell experiment [1]:

Cell lines

Peritoneal macrophages from wild-type and MyD88-deficient mice

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

100 nM

Applications

Unlike LPS-induced activation, which was MyD88-independent, Resiquimod-induced activation of NF-κB was completely dependent on MyD88. It was implied that Resiquimod activated macrophages via TLRs.

Animal experiment [1]:

Animal models

Wild-type, TLR7-deficient and MyD88-deficient mice

Dosage form

50 nmol; i.p.

Applications

In wild-type mice, Resiquimod (50 nmol, i.p.) increased serum concentrations of cytokines including IFN-alpha, TNF-alpha and IL-12. However, in TLR7-deficient and MyD88-deficient mice, there was not any increase in these cytokines.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Hemmi H, Kaisho T, Takeuchi O, Sato S, Sanjo H, Hoshino K, Horiuchi T, Tomizawa H, Takeda K, Akira S. Small anti-viral compounds activate immune cells via the TLR7 MyD88-dependent signaling pathway. Nat Immunol. 2002 Feb;3(2):196-200.

Quality Control