Puromycin dihydrochloride
Puromycin is an aminonucleoside antibiotic that induces premature chain termination during translation. This mechanism effectively inhibits protein synthesis, making puromycin a valuable tool for both in vivo and in vitro studies.
Puromycin rapidly inhibits the growth of bacteria, protozoa, and mammalian cells. However, cells expressing the pac gene, which is encoding a Puromycin N-acetyl-tranferase (PAC), are resistant. This enzyme inactivates puromycin by acetylation, preventing its interaction with the ribosome and conferring resistance. Although puromycin can be used to select resistant E. coli strains, it is more commonly employed for selecting mammalian cells.
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| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 544.43 |
| Cas No. | 58-58-2 |
| Formula | C22H29N7O5·2HCl |
| Solubility | ≥27.2 mg/mL in DMSO; ≥3.27 mg/mL in EtOH with ultrasonic; ≥99.4 mg/mL in H2O |
| Chemical Name | (S)-2-amino-N-((2S,3R,4S,5R)-5-(6-(dimethylamino)-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)-3-(4-methoxyphenyl)propanamide dihydrochloride |
| SDF | Download SDF |
| Canonical SMILES | O[C@H]1[C@H]([C@@H](CO)O[C@H]1N2C3=NC=NC(N(C)C)=C3N=C2)NC([C@H](CC(C=C4)=CC=C4OC)N)=O.Cl.Cl |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Cell experiment [1]: | |
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Cell lines |
T. thermophila cells |
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Preparation method |
The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months. |
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Reacting condition |
0 ~ 200 μg/mL; 0 ~ 72 hrs |
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Applications |
Puromycin Dihydrochloride, at the dose of 200 μg/mL, killed all T. thermophila cells by 48 hrs. At later time points, there was no survivors. |
| Animal experiment [2]: | |
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Animal models |
25-day- and 50-day- old mice |
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Dosage form |
0.2 mg/g; i.p. |
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Applications |
Puromycin Dihydrochloride, an autophagic inducer, elevated the level of free ribosomes. Up to 70% of cytoplasmic ribosomes were recovered in the free form, 30 to 60 mins after Puromycin Dihydrochloride treatment. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1]. Masaaki Iwamoto, ChieMori, Yasushi Hiraoka, et al. Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena. Gene, 2014, 534:249–255. [2]. Réz G, Kiss A, Bucsek MJ, Kovács J. Attachment of ribosomes to endoplasmic membranes in mouse pancreas. Degranulation in vivo caused by the inducers of autophagocytosis neutral red, vinblastine, puromycin, and cadmium ions, and prevention by cycloheximide. Chem Biol Interact. 1976 Apr;13(1):77-87. |
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Quality Control & MSDS
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Chemical structure
