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Puromycin dihydrochloride

Catalog No.
B7587
A selective antibiotic for pac gene
Grouped product items
SizePriceStock Qty
10mM (in 1mL H2O)
$60.00
In stock
20mg
$70.00
In stock
50mg
$140.00
In stock
For scientific research use only and should not be used for diagnostic or medical purposes.

Tel: +1-832-696-8203

Email: [email protected]

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Background

Puromycin is an aminonucleoside antibiotic that induces premature chain termination during translation. This mechanism effectively inhibits protein synthesis, making puromycin a valuable tool for both in vivo and in vitro studies. 
Puromycin rapidly inhibits the growth of bacteria, protozoa, and mammalian cells. However, cells expressing the pac gene, which is encoding a Puromycin N-acetyl-tranferase (PAC), are resistant. This enzyme inactivates puromycin by acetylation, preventing its interaction with the ribosome and conferring resistance. Although puromycin can be used to select resistant E. coli strains, it is more commonly employed for selecting mammalian cells.

Product Citation

Chemical Properties

Physical AppearanceA solid
StorageStore at -20°C
M.Wt544.43
Cas No.58-58-2
FormulaC22H29N7O5·2HCl
Solubility≥27.2 mg/mL in DMSO; ≥3.27 mg/mL in EtOH with ultrasonic; ≥99.4 mg/mL in H2O
Chemical Name(S)-2-amino-N-((2S,3R,4S,5R)-5-(6-(dimethylamino)-9H-purin-9-yl)-4-hydroxy-2-(hydroxymethyl)tetrahydrofuran-3-yl)-3-(4-methoxyphenyl)propanamide dihydrochloride
SDFDownload SDF
Canonical SMILESO[C@H]1[C@H]([C@@H](CO)O[C@H]1N2C3=NC=NC(N(C)C)=C3N=C2)NC([C@H](CC(C=C4)=CC=C4OC)N)=O.Cl.Cl
Shipping ConditionSmall Molecules with Blue Ice, Modified Nucleotides with Dry Ice.
General tips We do not recommend long-term storage for the solution, please use it up soon.

Protocol

Cell experiment [1]:

Cell lines

T. thermophila cells

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below - 20 °C for several months.

Reacting condition

0 ~ 200 μg/mL; 0 ~ 72 hrs

Applications

Puromycin Dihydrochloride, at the dose of 200 μg/mL, killed all T. thermophila cells by 48 hrs. At later time points, there was no survivors.

Animal experiment [2]:

Animal models

25-day- and 50-day- old mice

Dosage form

0.2 mg/g; i.p.

Applications

Puromycin Dihydrochloride, an autophagic inducer, elevated the level of free ribosomes. Up to 70% of cytoplasmic ribosomes were recovered in the free form, 30 to 60 mins after Puromycin Dihydrochloride treatment.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Masaaki Iwamoto, ChieMori, Yasushi Hiraoka, et al. Puromycin resistance gene as an effective selection marker for ciliate Tetrahymena. Gene, 2014, 534:249–255.

[2]. Réz G, Kiss A, Bucsek MJ, Kovács J. Attachment of ribosomes to endoplasmic membranes in mouse pancreas. Degranulation in vivo caused by the inducers of autophagocytosis neutral red, vinblastine, puromycin, and cadmium ions, and prevention by cycloheximide. Chem Biol Interact. 1976 Apr;13(1):77-87.

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