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Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO)

Catalog No.
K1024
Inhibit proteolytic degradation during bacterium lysis and protein extraction
Grouped product items
SizePriceStock Qty
1ml A + 1ml B
$35.00
In stock
10x(1ml A + 1ml B)
$250.00
In stock
50x(1ml A + 1ml B)
$1,000.00
In stock
100x(1ml A + 1ml B)
$1,500.00
In stock

Tel: +1-832-696-8203

Email: [email protected]

Worldwide Distributors

Description

Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain several endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.

Protease Inhibitor Cocktail for bacterial cell extracts (100 X in DMSO) is used with bacterium extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.

This protease inhibitor cocktail contains individual components, including AEBSF, Bestatin, E-64 and Pepstatin A with a broad specificity for cysteine, aminopeptidase, serine, aspartic, and metalloproteases. This cocktail has been optimized and tested for bacterium cell use. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.

Protocol

Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as bacterial cell extracts) before assaying.

Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.

Components and Storage

Tube Catalog No. Product Name Summary Targets CAS Number Smiles
A (100X in DMSO)
A2573 AEBSF.HCl Serine protease inhibitor Proteases|Serine Protease 30827-99-7 C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl
A2575 Bestatin Aminopeptidase inhibitor Proteases|Aminopeptidase 58970-76-6 CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576 E-64 Cysteine protease inhibitor,irreversible Proteases|Cathepsin 66701-25-5 CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2571 Pepstatin A Aspartic proteinases inhibitor Proteases|Other Proteases 26305-03-3 CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O
B (100X in ddH2O)
EDTA, disodium salt, dihydrate Metalloprotease inhibitor protease 6381-92-6
Stored at -20°C, and stable for at least 12 months.

Storage

Stored at -20°C, and stable for at least 12 months.