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Protease Inhibitor Cocktail (100X in DMSO, EDTA plus)

In stock
Catalog No.
K1019
Inhibit proteolytic degradation during cell lysis and protein extraction
Grouped product items
SizePriceStock Qty
1ml A + 1ml B
$29.00
In stock
20x(1ml A + 1ml B)
$420.00
In stock
50x(1ml A + 1ml B)
$945.00
In stock
100x(1ml A + 1ml B)
$1,420.00
In stock

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Protocol

Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc.

Components and storage

Tube Catalog No. Product Name Summary Targets CAS Number Smiles
A (100X in DMSO)
A2573 AEBSF.HCl Serine protease inhibitor Proteases|Serine Protease 30827-99-7 C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl
A2574 Aprotinin Inhibitor of bovine pancreatic trypsin Proteases|Serine Protease 9087-70-1
A2575 Bestatin Aminopeptidase inhibitor Proteases|Aminopeptidase 58970-76-6 CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O
A2576 E-64 Cysteine protease inhibitor,irreversible Proteases|Cathepsin 66701-25-5 CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O
A2570 Leupeptin Inhibitor of serine and cysteine proteases Proteases|Serine Protease 103476-89-7 O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H]
A2571 Pepstatin A Aspartic proteinases inhibitor Proteases|Other Proteases 26305-03-3 CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O
B (100X in ddH2O)
EDTA, disodium salt, dihydrate Metalloprotease inhibitor protease 6381-92-6
Stored at -20°C, and stable for at least 12 months.

Description

Protease Inhibitor Cocktail (100X in DMSO, EDTA plus), which is used during cell lysis and protein extraction, is a ready-to-use concentrated stock solutions of multiple protease inhibitors for endogenous protease. This product contains a separate 0.5M EDTA solution which inhibiting metalloproteases.

It generates optimized concentrations of six broad-spectrum protease inhibitors stabilized in DMSO, that powerfully inhibits serine-proteases, cysteine-proteases, aspartic acid-proteases and aminopeptidases appearing in cellular lysate samples. EDTA provides inhibition for metalloproteases.

EDTA inhibits metalloproteases by chelating the divalent cations that is necessary for their activity, so the activities of other proteins may be affected by EDTA. Therefore, empirical testing may be need in particular experiments to determine if EDTA will take a bad impact. If the protein of interest is to be purified using immobilized metal chelate affinity chromatography (IMAC) or analyzed by 2D gel electrophoresis, EDTA must be removed by extensive dialysis or desalting before purifying.