Protease Inhibitor Cocktail (100X in DMSO, EDTA plus)
This product is a protease inhibitor cocktail (100X in DMSO, EDTA plus), specifically designed for use during cell lysis and protein extraction processes. It is a ready-to-use concentrated solution that can inhibit a variety of endogenous proteases. Also included is a separate 0.5 M EDTA solution, which effectively inhibits metalloproteases.
The inhibitor cocktail consists of six carefully optimized broad-spectrum protease inhibitors dissolved in DMSO, capable of efficiently inhibiting serine proteases, cysteine proteases, aspartic proteases, and aminopeptidases commonly present in cell lysates. EDTA is provided for inhibition of metalloproteases. If immobilized metal affinity chromatography (IMAC) is involved for protein purification, or if two-dimensional (2D) gel electrophoresis analysis is planned, EDTA must be thoroughly removed by extensive dialysis or desalting prior to purification.
Specification: 1 Set equals 1X (1 mL A + 1 mL B), 10 Sets equals 10X (1 mL A + 1 mL B), and so on.
- 1. Ruiqi Qiu, Mingzhu Yang, et al. "AAV2-PDE6B restores retinal structure and function in the retinal degeneration 10 mouse model of retinitis pigmentosa by promoting phototransduction and inhibiting apoptosis." Neural Regen Res. 2025 Aug 1;20(8):2408-2419 PMID: 39359097
- 2. Min Shao, Haipeng Cheng, et al. "Abnormal mitochondrial iron metabolism damages alveolar type II epithelial cells involved in bleomycin-induced pulmonary fibrosis." Theranostics. 2024 Apr 15;14(7):2687-2705. PMID: 38773980
- 3. Liu L, Yin S, Gan W. "TRAF6 Promotes PRMT5 Activity in a Ubiquitination-Dependent Manner." Cancers, 2023, 15(9): 2501. PMID: 37173967
- 4. Yu Z, Wu Z, et al. "Structural basis of a two-step tRNA recognition mechanism for plastid glycyl-tRNA synthetase." Nucleic Acids Research, 2023, 51(8): 4000-4011. PMID: 36912079
- 5. Reham Alsaadi. "Characterization of post-translational modification of ATG16L1 in antibacterial autophagy." University of Ottawa. 2019.
| Thaw on ice, add at 1:100 (v/v) dilution to solution samples (such as cell lysates or tissue extracts) before assaying. |
| Applications: WB, Co-IP, pull-down, IF, IHC, Flow Cytometry, kinase assay and etc. |
| Tube | Catalog No. | Product Name | Summary | Targets | CAS Number | Smiles |
| A (100X in DMSO) | ||||||
| A2573 | AEBSF.HCl | Serine protease inhibitor | Proteases|Serine Protease | 30827-99-7 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl | |
| A2574 | Aprotinin | Inhibitor of bovine pancreatic trypsin | Proteases|Serine Protease | 9087-70-1 | ||
| A2575 | Bestatin | Aminopeptidase inhibitor | Proteases|Aminopeptidase | 58970-76-6 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O | |
| A2576 | E-64 | Cysteine protease inhibitor,irreversible | Proteases|Cathepsin | 66701-25-5 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O | |
| A2570 | Leupeptin | Inhibitor of serine and cysteine proteases | Proteases|Serine Protease | 103476-89-7 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] | |
| A2571 | Pepstatin A | Aspartic proteinases inhibitor | Proteases|Other Proteases | 26305-03-3 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O | |
| B (100X in ddH2O) | ||||||
| EDTA, disodium salt, dihydrate | Metalloprotease inhibitor | protease | 6381-92-6 | |||
| Stored at -20°C, and stable for at least 12 months. | ||||||