In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Limonin is a chemically-induced carcinogenesis inhibitor and HIV-1 replication inhibitor.The human immunodeficiency virus (HIV) is a lentivirus that causes the acquired immunodeficiency syndrome (AIDS).
When culturing the HIV-1 in human peripheral blood mononuclear cells (PBMC) and monocytes/macrophages (M/M), Limonin inhibit the HIV-1 replication in all cellular systems and inhibit HIV-1 replication with EC50 value of 60u μM in PBMC cells in a dose-dependent way . In human hepatoma HepG2 cells, limonin can induced apoptosis, which was evidenced by WB analysis of some apoptosis factors .
In the female Syrian hamsters treated with DMBA, which can cause tumor, limonin showed a 60% reduction in tumor burden. It decreased 20% tumor number and 50% tumor mass . In male F344 rats treated with azoxymethane (AOM), 0.05% limonin in diet significantly reduced (65%-92% inhibition) the incidence of colonic adenocarcinoma. Thus, limonin has chemopreventive effects on chemically induced carcinogenesis .
. Battinelli L, Mengoni F, Lichtner M, et al. Effect of limonin and nomilin on HIV-1 replication on infected human mononuclear cells. Planta medica, 2003, 69(10): 910-913.
. Langeswaran K, Gowthamkumar S, Vijayaprakash S, et al. Influence of limonin on Wnt signalling molecule in HepG2 cell lines. J Nat Sci Biol Med, 2013, 4(1): 126-133.
. Miller EG, Fanous R, Rivera-Hidalgo, et al. The effect of citrus limonoids on hamster buccal pouch carcinogenesis. Carcinogenesis, 1989, 10(8): 1535-1537.
. Tanaka T, Kohno H, Tsukio Y, et al. Citrus limonoids obacunone and limonin inhibit azoxymethane-induced colon carcinogenesis in rats. BioFactors (Oxford, England), 2000, 13(1-4): 213-8.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Synonyms||Limonoic acid 3,19:16,17 dilactone|
|Solubility||≥23.9 mg/mL in DMSO; insoluble in H2O; insoluble in EtOH|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|