Large T antigen - rhesus polyomavirus 560-568
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Large T antigen - rhesus polyomavirus 560-568 has a peptide sequence of Ser-Glu-Phe-Leu-Leu-Glu-Lys-Arg-Ile.
T antigen is required for viral DNA replication, transcription, and virion assembly. In addition, T antigen targets multiple cellular pathways, including those that regulate cell proliferation, cell death, and the inflammatory response. The large T-antigen plays a key role in regulating the viral life cycle by binding to the viral origin of DNA replication where it promotes DNA synthesis. Also as the polyomavirus relies on the host cell machinery to replicate the host cell needs to be in s-phase for this to begin. Due to this, large T-antigen also modulates cellular signaling pathways to stimulate progression of the cell cycle by binding to a number of cellular control proteins.
Polyomaviruses have been extensively studied as tumor viruses in humans and animals, leading to fundamental insights into carcinogenesis, DNA replication and protein processing. The tumor suppressor molecule p53 was discovered, for example, as a cellular protein bound by the major oncoprotein (cancer-causing protein) T antigen made by Simian vacuolating virus 40 (SV40).
Figure 1: Genome of the polyomaviruses
1. An P, Saenz Robeles MT, Pipas JM. “Large T antigens of polyomaviruses: amazing molecular machines”. Annu Rev Microbiol. 2012; 66:213-36.
2. White MK, Gordon J, Reiss K, et al. (December 2005). "Human polyomaviruses and brain tumors". Brain Research. Brain Research Reviews 50 (1): 69–85.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥113.4 mg/mL in DMSO; insoluble in EtOH; ≥23.13 mg/mL in H2O|
|Canonical SMILES||N[[email protected]](C(N[[email protected]@H](CCC(O)=O)C(N[[email protected]@H](CC1=CC=CC=C1)C(N[[email protected]@H](CC(C)C)C(N[[email protected]@H](CC(C)C)C(N[[email protected]@H](CCC(O)=O)C(N[[email protected]@H](CCCCN)C(N[[email protected]@H](CCCNC(N)=N)C(N[[email protected]@H]([[email protected]@H](C)CC)C(O)=O)=O)=O)=O)=O)=O)=O)=O)=O)CO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|