Digoxigenin-11-UTP
Digoxigenin-11-UTP has been used for labeling RNA probes for in situ hybridization and other purposes. DIG-11-UTP is a substrate for T7, SP6 and T3 RNA polymerases* and can replace UTP in in vitro transcription for DIG-labeling of RNA in a ratio of 35%: 65%.
Linearized template DNA with T7, SP6 or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP and DIG-11-UTP, respectively.
Labeled RNA can be subsequently detected with the DIG Luminescent Detection Kit for Nucleic Acids* or anti-DIG-AP* and CDP-Star*.
References:
[1] Mugford S, et al., A Serine Carboxypeptidase-Like Acyltransferase Is Required for Synthesis of Antimicrobial Compounds and Disease Resistance in Oats, The Plant Cell 21:2473-2484 (2009)
[2]-Pontes O,et al., Natural variation in nucleolar dominance reveals the relationship between nucleolus organizer chromatin topology and rRNA gene transcription in Arabidopsis, PNAS vol. 100 no. 20 11418-11423 (2003)
[3]Schaeren-Wiemers N.et al., A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes, Histochemistry and Cell Biology, Volume 100, Number 6 (1993)
[4]Yanyan L. et al., PiggyBac transgenic strategies in the developing chicken spinal cord, Nucleic Acids Res., Sep 2009;10.1093/nar/gkp686
[5]Zwirglmaier K.et al., In situ functional gene analysis: recognition of individual genes by fluorescence in situ hybridization, Methods Enzymol. 397:338-51 (2005)
| Physical Appearance | Solution |
| Storage | Store at -20°C or below |
| M.Wt | 1082.92 (free acid) |
| Formula | C43H65N4O22P3 (free acid) |
| Synonyms | Digoxigenin-X-5-aminoallyl-uridine-5’-triphosphate, Triethylammonium salt, Dig-11-utp |
| Solubility | sterile clear aqueous solution, in 100 mM Tris-HCl pH 7.5 ±0.5 |
| Chemical Name | ((2R,3S,4R,5R)-5-(5-((E)-3-(6-(2-(((3S,5R,8R,9S,10S,12R,13S,14S,17R)-12,14-dihydroxy-10,13-dimethyl-17-(5-oxo-2,5-dihydrofuran-3-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)acetamido)hexanamido)prop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H |
| SDF | Download SDF |
| Canonical SMILES | O=C1OCC([C@H]2CC[C@@]3(O)[C@]2(C)[C@H](O)C[C@@]4([H])[C@@]3([H])CC[C@@]5([H])[C@]4(C)CC[C@H](OCC(NCCCCCC(NC/C=C/C6=CN([C@H]7[C@H](O)[C@H](O)[C@@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O7)C(NC6=O)=O)=O)=O)C5)=C1 |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
Quality Control & MSDS
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Purity = 98.00%
- COA (Certificate Of Analysis)
- MSDS (Material Safety Data Sheet)
Chemical structure
