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Digoxigenin-11-UTP

Catalog No.
B7953
Used in transcriptional labeling of RNA probe for in situ hybridization and other purposes
Grouped product items
SizePriceStock Qty
25ul (10 mM)
$240.00
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Tel: +1-832-696-8203

Email: [email protected]

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Background

Digoxigenin-11-UTP has been used for labeling RNA probes for in situ hybridization and other purposes. DIG-11-UTP is a substrate for T7, SP6 and T3 RNA polymerases* and can replace UTP in in vitro transcription for DIG-labeling of RNA in a ratio of 35%: 65%.

Linearized template DNA with T7, SP6 or T3 promoter is in vitro transcribed with the corresponding RNA polymerases using ATP, GTP, CTP, UTP and DIG-11-UTP, respectively.

Labeled RNA can be subsequently detected with the DIG Luminescent Detection Kit for Nucleic Acids* or anti-DIG-AP* and CDP-Star*.

References:
[1] Mugford S, et al., A Serine Carboxypeptidase-Like Acyltransferase Is Required for Synthesis of Antimicrobial Compounds and Disease Resistance in Oats, The Plant Cell 21:2473-2484 (2009)
[2]-Pontes O,et al., Natural variation in nucleolar dominance reveals the relationship between nucleolus organizer chromatin topology and rRNA gene transcription in Arabidopsis, PNAS vol. 100 no. 20 11418-11423 (2003)
[3]Schaeren-Wiemers N.et al., A single protocol to detect transcripts of various types and expression levels in neural tissue and cultured cells: in situ hybridization using digoxigenin-labelled cRNA probes, Histochemistry and Cell Biology, Volume 100, Number 6 (1993)
[4]Yanyan L. et al., PiggyBac transgenic strategies in the developing chicken spinal cord, Nucleic Acids Res., Sep 2009;10.1093/nar/gkp686
[5]Zwirglmaier K.et al., In situ functional gene analysis: recognition of individual genes by fluorescence in situ hybridization, Methods Enzymol. 397:338-51 (2005)

Chemical Properties

Physical AppearanceSolution
StorageDesiccate at -20°C
M.Wt1082.92 (free acid)
FormulaC43H65N4O22P3 (free acid)
SynonymsDigoxigenin-X-5-aminoallyl-uridine-5’-triphosphate, Triethylammonium salt, Dig-11-utp
Solubilitysterile clear aqueous solution, in 100 mM Tris-HCl pH 7.5 ±0.5
Chemical Name((2R,3S,4R,5R)-5-(5-((E)-3-(6-(2-(((3S,5R,8R,9S,10S,12R,13S,14S,17R)-12,14-dihydroxy-10,13-dimethyl-17-(5-oxo-2,5-dihydrofuran-3-yl)hexadecahydro-1H-cyclopenta[a]phenanthren-3-yl)oxy)acetamido)hexanamido)prop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H
SDFDownload SDF
Canonical SMILESO=C1OCC([[email protected]]2CC[[email protected]@]3(O)[[email protected]]2(C)[[email protected]](O)C[[email protected]@]4([H])[[email protected]@]3([H])CC[[email protected]@]5([H])[[email protected]]4(C)CC[[email protected]](OCC(NCCCCCC(NC/C=C/C6=CN([[email protected]]7[[email protected]](O)[[email protected]](O)[[email protected]@H](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)O7)C(NC6=O)=O)=O)=O)C5)=C1
Shipping ConditionEvaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.
General tipsFor obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.

Quality Control

Quality Control & MSDS

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Chemical structure

Digoxigenin-11-UTP