Curcumin
mRNA synthesis
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Inhibitor Cocktails
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Curcumin is an inhibitor of tyrosinase with IC50 value of 47uM [1].
Curcumin is a natural compound with potency of anticancer. It is currently under clinical investigation for cancer chemoprevention. There is a variety of biochemical mechanisms of this anticancer function. The targets of Curcumin are involved in signaling pathways include transcription factors, growth factors,
inflammatory cytokines, receptors, and enzymes. Phase I trials have tested the toxicity and tolerability of Curcumin and found that Curcumin has no toxicities at doses up to 12 g/day. However, the bioavailability of Curcumin is quite poor. It is about ~1% after oral administration in phase I/II clinical trials and this hinders Curcumin‘s use in the clinic [2].
It is also reported that Curcumin reduces the generation of amloid beta (Aβ 40 and Aβ42) in SH-SY5Y neuroblastoma cells. It also down-regulates the expression of PS1 and GSK-3β in cells. All these cause the inhibition of Aβ formation and make Curcumin to be a potent therapeutic agent in AD [3].
References:
[1] Sachiko Shirota, Kouji Miyazaki, Ritsuo Aiyama, Minoru Ichioka and Teruo Yokokura. Tyrosinase inhibitors from crude drugs. Biol. Pharm. Bull. 1994, 17 (2): 266-269.
[2] Wungki Park, A.R.M Ruhul Amin, Zhuo Georgia Chen, and Dong M. Shin. New perspectives of curcumin in cancer prevention. Cancer Prev Res (Phila). 2013, 6(5): 387–400.
[3] Zhang Xiong, Zhang Hongmei, SiLu, LiYu. Curcumin mediates presenilin-1 activity to reduce β-amyloid production in a model of Alzheimer’s disease. Pharmacological Reports. 2013, 63: 1101-1108.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 368.38 |
| Cas No. | 458-37-7 |
| Formula | C21H20O6 |
| Synonyms | Indian Saffron;Turmeric yellow;Natural Yellow 3;Diferuloylmethane |
| Solubility | ≥36.8 mg/mL in DMSO; insoluble in H2O; ≥3.5 mg/mL in EtOH with gentle warming and ultrasonic |
| Chemical Name | (1E,6E)-1,7-bis(4-hydroxy-3-methoxyphenyl)hepta-1,6-diene-3,5-dione |
| SDF | Download SDF |
| Canonical SMILES | COC1=C(C=CC(=C1)C=CC(=O)CC(=O)C=CC2=CC(=C(C=C2)O)OC)O |
| Shipping Condition | Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request. |
| General tips | For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
| Cell experiment:[1] | |
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Cell lines |
B16-R melanoma cells resistant to doxorubicin |
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Reaction Conditions |
1 ~ 200 μM curcumin for 24, 36 or 48 h incubation |
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Applications |
Curcumin was found to be cytotoxic in vitro for B16-R melanoma cells resistant to doxorubicin either cultivated as monolayers (1 ~ 100 μM) or grown in three-dimensional cultures (1 ~ 200 μM). The cytotoxic effect observed in the 2 culture types was related to the induction of programmed cell death. |
| Animal experiment:[1] | |
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Animal models |
Female B6D2F1 mice (6 ~ 8 weeks old) challenged subcutaneously with B16-R melanoma cells |
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Dosage form |
25 mg/kg Once daily by intraperitoneal injection |
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Applications |
The combination treatment consisting of curcumin and soluble B16-R proteins resulted in substantial inhibition of growth of B16-R melanoma, whereas each treatment by itself showed little effect. Moreover, animals receiving the combination therapy exhibited an enhancement of their humoral anti-soluble B16-R protein immune response and a significant increase in their median survival time. Therefore, curcumin may provide a valuable tool for the development of a therapeutic combination against the melanoma. |
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Note |
The technical data provided above is for reference only. |
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References: 1. Odot J, Albert P, Carlier A, et al. In vitro and in vivo anti-tumoral effect of curcumin against melanoma cells. International Journal of Cancer, 2004, 111(3): 381-387. |
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Quality Control & MSDS
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Chemical structure
