5-BrdU
5-Bromo-2'-deoxyuridine (5-BrdU; CAS 59-14-3) is a thymidine analog that integrates into DNA during S-phase of cell division. Its incorporation facilitates immunodetection via specific monoclonal or polyclonal antibodies. In vitro studies show that early application of 5-BrdU to Nicotiana glauca pith explants inhibits cellular proliferation, an effect reversed by addition of thymidine or deoxycytidine. In vivo, BrdU immunostaining identifies proliferating neuronal cells in mouse central nervous system tissues, paralleling earlier thymidine labeling studies. Thus, 5-BrdU serves as a molecular tool for analyzing cell cycle progression and neurogenesis in biomedical research.
| Physical Appearance | A solid |
| Storage | Store at -20°C |
| M.Wt | 307.1 |
| Cas No. | 59-14-3 |
| Formula | C9H11BrN2O5 |
| Solubility | insoluble in EtOH; ≥11.56 mg/mL in 0.9% NS with ultrasonic; ≥15.35 mg/mL in DMSO; ≥16.23 mg/mL in H2O with ultrasonic |
| Chemical Name | 5-bromo-1-((2R,4S,5R)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)pyrimidine-2,4(1H,3H)-dione |
| SDF | Download SDF |
| Canonical SMILES | BrC(C(N1)=O)=CN(C1=O)[C@@]2([H])C[C@@](O)([H])[C@@](O2)([H])CO |
| Shipping Condition | Small Molecules with Blue Ice, Modified Nucleotides with Dry Ice. |
| General tips | We do not recommend long-term storage for the solution, please use it up soon. |
| Vitro experiment [1]: | |
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Samples |
Nicotiana pith tissues |
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Preparation method |
The solubility of this compound in DMSO is >15.4 mg/mL. General tips for obtaining a higher concentration: Please warm the tube at 37°C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months. |
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Reacting condition |
5 μg/mL; 24 h |
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Applications |
In nicotiana pith tissues, BrdU inhibited growth of pith explants, while thymidine completely reversed the inhibitory effect. The inhibition observed could be attributed to BrdU incorporation into DNA. |
| Animal experiment [2]: | |
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Animal models |
Pregnant NMRI mice |
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Dosage form |
25 mg/kg (5 mg/mL dissolved in 0.1 M Tris-buffered saline, pH 7.6), i.p.; between embryonic days E11 and E17. |
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Application |
In pregnant NMRI mice, a single pulse of 5-bromodeoxyuridine (BrdU) was injected between embryonic days E11 and E17 to label postmitotic neuroblasts. BrdU is an analog of Thy that is incorporated into the DNA of cells undergoing the S-phase and can be detected by polyclona or monoclonal antibodies. Immunoreactive nuclei were detected in the adult CNS after pulses of BrdU. BrdU could be used to map neuronal birthdates. |
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Other notes |
Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal. |
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References: [1] M. Durante, C. Geri, V. Nuti-Ronchi, G. Martini, E. Guillé, J. Grisvard, L. Giorgi, R. Parenti, M. Bulatti. Inhibition of Nicotiana glauca pich tissue proliferation through incorporation of 5-BrdU into SNA. Cell Differentiation, Volume 6, Issue 1, June 1977, Pages 53–63 [2] del Rio JA, Soriano E. Immunocytochemical detection of 5'-bromodeoxyuridine incorporation in the central nervous system of the mouse. Brain Res Dev Brain Res. 1989 Oct 1; 49 (2): 311-7. |
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