In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Aminoallyl-UTP is used for Nucleic Acid Sequence Based Amplification (NASBA), Microarray based hybridization detection, Eberwine mRNA amplification and RNA-labeling. It is enzymatically incorporated into RNA during the NASBA reaction and Eberwine mRNA amplification. The resulting amine-functionalized RNA can subsequently be labeled via the classic Amine/NHS Ester reaction that introduces fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microarray based hybridization detection.
 Scheler et al. (2009) Fluorescent labeling of NASBA amplified tmRNA molecules for microarray applications. BMC Biotechnol. 9:45.
 Hoen et al. (2003) Fluorescent labelling of cRNA for microarray applications. Nucleic Acids Res. 31 (5):e20.
|Storage||Store at -20°C|
|Solubility||Soluble in sterile water|
|Chemical Name||((2R,3S,4R,5R)-5-(5-((E)-3-aminoprop-1-en-1-yl)-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)-3,4-dihydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate|
|Canonical SMILES||O=C(N1)N([[email protected]@H]2O[[email protected]](COP(O)(OP(O)(OP(O)(O)=O)=O)=O)[[email protected]@H](O)[[email protected]]2O)C=C(/C=C/CN)C1=O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|