vitamin D binding protein precursor (353-363) [Homo sapiens]
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Human vitamin D-binding protein (hDBP),' also known as Gcglobulin, is an abundant, multifunctional, and highly polymorphic serum glycoprotein synthesized by the liver1. DBP is the major serum transport protein for the vitamin D sterols2, binds and sequesters monomers of actin with high affinity3, 4, and has been identified on the surface of a variety of cell types including B-lymphocytes5, subpopulations of T-lymphocytes6, and the cytotrophoblasts of the placenta7.
DBP is a secreted protein and would therefore be predicted to have a signal sequence. The amino-terminus of the mature serum protein was established by aligning the sequence predicted by the cDNA to a sequenced amino-terminal hDBP peptide8.
Figure1. Structure of Vitamin D binding protein
Figure2. Formula of Vitamin D binding protein precursor (353-363)
1.Haddad, J. G. 1979. Transport of vitamin D metabolites. Clin. Orthop. Relat. Res. 142:249-274.
2.Daiger, S. P., M. S. Schanfield, and L. L. Cavalli-Sforza. 1975. Group specific component (Gc) proteins bind vitamin D and 25-hydroxyvitamin D. Proc. Natl. Acad. Sci. USA. 72:2076-2080.
3.Cooke, N. E., J. Walgate, and J. G. Haddad. 1979. Human serum binding protein for vitamin D and its metabolites. I. Physicochemical and immunological identification in human tissues. J. Biol. Chem. 254: 5965-5971.
4.Van Baelen, H., R. Bouillon, and P. DeMoor. 1980. Vitamin D binding protein (Gc-Globulin) binds actin. J. Biol. Chem. 255:2270-2272.
5.Petrini, M., D. L. Emerson, and R. M. Galbraith. 1983. Linkage between surface immunoglobulin and cytoskeleton of B lymphocytes may involve Gc protein. Nature (Lond.). 306:73-74.
6.Petrini, M., R. M. Galbraith, D. L. Emerson, A. E. Nel, and P. Arnaud. 1985. Structural studies of T lymphocyte Fc receptors. J. Biol. Chem. 260:1804-1810.
7.Nestler, J. E., E. Lange, J. McLeod, H. J. Kliman, and J. F. Strauss. 1985. Detection of vitamin D binding protein (DBP) in human cytotrophoblasts. Seventh Annual American Societyfor Bone and Mineral Research. Wash. DC. 266a. (Abstr.).
8.Svasti, J., A. Kurosky, A. Bennett, and B. H. Bowman. 1979. Molecular basis for the three major forms of human serum vitamin D binding protein (group specific component). Biochemistry. 18:1611-1617.
|Physical Appearance||A solid|
|Storage||Store at -20°C|
|Solubility||≥132.7mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|