In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Trimidox (3.4.5-tri-hydroxybenzohydroxamidoxime) is a specific and patented ribonucleotide reductase (RNR) inhibitor .
Ribonucleotide reductase (RNR), also known as ribonucleoside diphosphate reductase, is an enzyme involved in catalyzing the formation of deoxyribonucleotides from ribonucleotides. RNR plays a vital role in regulating the total rate of DNA synthesis to DNA to maintain DNA cell mass a constant ratio during cell division and DNA repair. The substrates for RNR are ADP, GDP, CDP and UDP . Increased ribonucleotide reductase (RNR) activity has been associated with malignant transformation and tumor cell growth .
In human promyelocytic leukemia HL-60 cells, trimidox inhibited the growth of with an IC50 of 35 pmol/L. Treatment with trimidox (50 pmol/L) for 24 hours decreased deoxyguanosine triphosphate (dGTP) and deoxycytidine triphosphate (dCTP) pools to 24% and 3946 of control values, respectively. Incubation of HL-60 cells with 20 to 80 pmol/L trimidox to 4 days showed no effects in the distribution of cells indifferent phases of cell cycle . Trimidox in combination with tiazofurin was useful in the treatment of leukemia .
 Szekeres T, Fritzer M, Strobl H, et al. Synergistic growth inhibitory and differentiating effects of trimidox and tiazofurin in human promyelocytic leukemia HL-60 cells[J]. Blood, 1994, 84(12): 4316-4321.
 Elledge S J, Zhou Z, Allen J B. Ribonucleotide reductase: regulation, regulation, regulation[J]. Trends in biochemical sciences, 1992, 17(3): 119-123.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||≤20mg/ml in DMSO;20mg/ml in dimethyl formamide|
|Shipping Condition||Ship with blue ice, or upon other requests.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. We do not recommend long-term storage for the solution, please use it up soon.|