Tin protoporphyrin IX dichloride
In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tin protoporphyrin IX dichloride is a synthetic heme analog that selectively inhibits heme oxygenase 2 (HO-2) with IC50 value of 7.5 µM .
HO-2, widely expressed with high concentrations in the brain, is one of two forms of HO (HO-1 and HO-2). HO is responsible for converting heme to biliverdin, and carbon monoxide (CO), which has been implicated as a biological messenger molecule analogous to nitric oxide (NO) with endothelial-derived relaxing activity .
Tin protoporphyrin IX dichloride is a selective inhibitor of HO with ~ 10-fold selectivity for HO over endothelial nitric oxide synthase (NOS) and soluble guanylyl cyclase (IC50s = 35 and 30 nM, respectively). Tin protoporphyrin IX dichloride (1 ~ 100 µM) reduced acetylcholine (Ach)-induced endothelial-derived relaxation of porcine distal pulmonary arteries in the presence of Nw-nitro-Larginine methyl ester (L-NAME), with an IC50 of 7.6 µM .
In Sprague-Dawley rat neonates, a single subcutaneous injection of Tin protoporphyrin IX dichloride (100 µmol/kg) at birth, 12, 24, 48, and 72 hr later, blocked the postnatal increase in heme oxygenase activity that occurs in various tissues. Tin protoporphyrin IX dichloride administration also entirely prevented the development of hyperbilirubinemia that normally occurs postnatally .
. Zakhary R, Gaine S P, Dinerman J L, et al. Heme oxygenase 2: Endothelial and neuronal localization and role in endothelium-dependent relaxation. Proceedings of the National Academy of Sciences of the United States of America, 1996, 93(2): 795-798.
. Drummond G S, Kappas A. Prevention of neonatal hyperbilirubinemia by tin protoporphyrin IX, a potent competitive inhibitor of heme oxidation. Proceedings of the National Academy of Sciences of the United States of America, 1981, 78(10): 6466-6470.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||insoluble in EtOH; insoluble in H2O; ≥22.15 mg/mL in DMSO|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|