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Thapsigargin
sarco-endoplasmic reticulum Ca2+-ATPases inhibitor

Catalog No.B6614
Size Price Stock Qty
1mg
$109.00
In stock

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Sample solution is provided at 25 µL, 10mM.

Product Citations

1. Chou CK, Liu W, et al. "Ethyl Acetate Extract of Scindapsus cf. hederaceus Exerts the Inhibitory Bioactivity on Human Non-Small Cell Lung Cancer Cells through Modulating ER Stress." Int J Mol Sci. 2018 Jun 21;19(7). pii: E1832. PMID:29933620

Quality Control

Quality Control & MSDS

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Chemical structure

Thapsigargin

Related Biological Data

Thapsigargin

Protocol

Cell experiment [1,2]:

Cell lines

NG115-401L neural cell line, isolated rat hepatocytes

Preparation method

The solubility of this compound in DMSO is > 10 mM. General tips for obtaining a higher concentration: Please warm the tube at 37 ℃ for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20℃ for several months.

Reacting condition

ED50: ~20 nM (NG115-401L neural cell line); ED50 ~80 nM (isolated rat hepatocytes)

Applications

Thapsigargin stimulated a rapid (within 15 s) transient increase in intracellular [Ca2+] in the NG115-401L neural cell line with the ED50 of around 20 nM. In isolated rat hepatocytes, Thapsigargin increased [Ca2+] in a rapid and dose-dependent manner, with an ED50 value of ~80 nM.

Animal experiment [3]:

Animal models

Male C57BL/6 mice with transient middle cerebral artery occlusion (tMCAO)

Dosage form

IC50: 2 to 20 ng, icv injection

Application

Thapsigargin dose-dependently reduced the brain infarct. Thapsigargin (TG) protected mice against ischemia-reperfusion-induced brain injury.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Jackson T R, Patterson S I, Thastrup O, et al. A novel tumour promoter, thapsigargin, transiently increases cytoplasmic free Ca2+ without generation of inositol phosphates in NG115-401L neuronal cells[J]. Biochemical Journal, 1988, 253(1): 81-86.

[2]. Thastrup O, Cullen P J, Drbak B K, et al. Thapsigargin, a tumor promoter, discharges intracellular Ca2+ stores by specific inhibition of the endoplasmic reticulum Ca2 (+)-ATPase[J]. Proceedings of the National Academy of Sciences, 1990, 87(7): 2466-2470.

[3]. Zhang X, Yuan Y, Jiang L, et al. Endoplasmic reticulum stress induced by tunicamycin and thapsigargin protects against transient ischemic brain injury: Involvement of PARK2-dependent mitophagy[J]. Autophagy, 2014, 10(10): 1801-1813.

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Chemical Properties

Cas No. 67526-95-8 SDF Download SDF
Synonyms N/A
Chemical Name (3S,3aR,4S,6S,6aR,7S,8S,9bS)-6-acetoxy-4-(butyryloxy)-3,3a-dihydroxy-3,6,9-trimethyl-8-(((Z)-2-methylbut-2-enoyl)oxy)-2-oxo-2,3,3a,4,5,6,6a,7,8,9b-decahydroazuleno[4,5-b]furan-7-yl octanoate
Canonical SMILES O[[email protected]@]([[email protected]]1OC(CCC)=O)([[email protected]]2(C)O)[[email protected]](C([[email protected]]([[email protected]@H]3OC(CCCCCCC)=O)[[email protected]@](C1)(C)OC(C)=O)=C(C)[[email protected]@H]3OC(/C(C)=C\C)=O)OC2=O
Formula C34H50O12 M.Wt 650.76
Solubility Soluble in DMSO > 10 mM Storage Desiccate at -20°C
Physical Appearance A crystalline solid Shipping Condition Evaluation sample solution : ship with blue ice.All other available size: ship with RT , or blue ice upon request
General tips For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months.