In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
Tetracycline Hydrochloride is an inhibitor of bacterial ribosomes .
Tetracycline Hydrochloride is believed to inhibit translation by binding to the 16S rRNA and inhibiting the binding of aminoacyl-tRNA to the mRNA-ribosome complex. However, the exact target site(s) and mechanism(s) of action remain subjects of much debate .
Tetracycline Hydrochloride at doses of 0, 10, 25, 50, 75, 150, 225 and 300 µM exhibited increasing antimicrobial effect against resistant strains of Staphylococcus aureus (S. aureus). The statistically evaluated IC50 values of Tetracycline Hydrochloride against S. aureus, cadmium-resistant S. aureus, and lead-resistant S. aureus after first 6 h were 4.8, 3.8, 2.2 µM, respectively .
In humans who had minimum Propionibacterium acnes (P. acnes) levels of 10000 colony-forming units per cm2 at baseline, Tetracycline Hydrochloride treatment (1000 mg/d, b.i.d., p.o.) over a 6-week period significantly reduced P. acnes on both cheeks and forehead after 3 and 6 weeks, but produced little to no residual effect. At 9 weeks, P. acnes levels in the Tetracycline Hydrochloride group had returned to baseline .
. Chukwudi C U. rRNA Binding Sites and the Molecular Mechanism of Action of the Tetracyclines. Antimicrobial Agents and Chemotherapy, 2016, 60(8): 4433-4441.
. Chudobova D, Dostalova S, Blazkova I, et al. Effect of ampicillin, streptomycin, penicillin and tetracycline on metal resistant and non-resistant Staphylococcus aureus. International Journal of Environmental Research and Public Health, 2014, 11(3): 3233-3255.
. Leyden J J, Kaidbey K, Gans E H. The antimicrobial effects in vivo of minocycline, doxycycline and tetracycline in humans. Journal of Dermatological Treatment, 1996, 7: 223-225.
|Storage||Store at -20°C|
|Solubility||≥12.02 mg/mL in DMSO with gentle warming, ≥57.7 mg/mL in H2O|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|
Quality Control & MSDS
- View current batch:
Purity = 98.03%
- COA (Certificate Of Analysis)
- NMR (Nuclear Magnetic Resonance)
- MSDS (Material Safety Data Sheet)