In vitro transcription of capped mRNA with modified nucleotides and Poly(A) tail
Tyramide Signal Amplification (TSA)
TSA (Tyramide Signal Amplification), used for signal amplification of ISH, IHC and IC etc.
Phos Binding Reagent Acrylamide
Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody
Cell Counting Kit-8 (CCK-8)
A convenient and sensitive way for cell proliferation assay and cytotoxicity assay
SYBR Safe DNA Gel Stain
Safe and sensitive stain for visualization of DNA or RNA in agarose or acrylamide gels.
Protect the integrity of proteins from multiple proteases and phosphatases for different applications.
STO-609 acetate is a specific inhibitor of the Ca2+/calmodulin-dependent protein kinase kinase (CaM-KK), with Ki values of 80 and 15 ng/ml against CaM-KKα and CaM-KKβ isoforms, respectively .
CaM-KKα and CaM-KKβ, highly expressed in the brain with the α isoform also expressed in various peripheral tissues such as thymus and spleen in mammals, belongs to Ca2+/calmodulin-dependent protein kinases, which are involved in many cellular responses mediated by an increase in the concentration of intracellular calcium .
STO-609 exhibited high selectivity for CaM-KK without significantly affecting the downstream CaM kinases (CaM-KI and -IV), and the IC50 value of STO-609 against CaM-KII was ~ 10 μg/ml. In transfected HeLa cells, STO-609 (0.01 ~ 10 μg/ml) suppressed the Ca2+-induced activation of CaM-KIV in a dose-dependent manner. In addition, in SH-SY5Y neuroblastoma cells, STO-609 at a concentration of 1 μg/ml significantly reduced the endogenous activity of CaM-KK .
In ovariectomized mice, in vivo administration of STO-609 (10 μM, 200 μl per mouse, i.p., thrice a week) resulted in increased osteoblasts and diminished osteoclasts, significantly protect adult mice from ovariectomy-induced osteoporosis .
. Tokumitsu H, Inuzuka H, Ishikawa Y, et al. STO-609, a specific inhibitor of the Ca(2+)/calmodulin-dependent protein kinase kinase. Journal of Biological Chemistry, 2002, 277(18): 15813-15818.
. Cary R L, Waddell S, Racioppi L, et al. Inhibition of Ca²⁺/calmodulin-dependent protein kinase kinase 2 stimulates osteoblast formation and inhibits osteoclast differentiation. Journal of Bone and Mineral Research, 2013, 28(7): 1599-1610.
|Physical Appearance||A crystalline solid|
|Storage||Store at -20°C|
|Solubility||Soluble in DMSO|
|Chemical Name||7-oxo-7H-benzo[de]benzo[4,5]imidazo[2,1-a]isoquinoline-3-carboxylic acid compound with acetic acid (1:1)|
|Shipping Condition||Evaluation sample solution: ship with blue ice. All other available sizes: ship with RT, or blue ice upon request.|
|General tips||For obtaining a higher solubility, please warm the tube at 37°C and shake it in the ultrasonic bath for a while. Stock solution can be stored below -20°C for several months.|